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Int. J. Mol. Sci. 2015, 16(4), 7045-7056; doi:10.3390/ijms16047045

Rapid Characterization of Fatty Acids in Oleaginous Microalgae by Near-Infrared Spectroscopy

1,2
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2,3
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1,2
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4
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1
and
2,5,*
1
School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China
2
Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China
3
Institute of Marine and Environmental Technology, University of Maryland Center for Environmental Science, Baltimore, MD 21202, USA
4
The School of Food Science and Technology, Jiangnan University, Wuxi 214122, China
5
Singapore-Peking University Research Centre for a Sustainable Low-Carbon Future, CREATE Tower 138602, Singapore
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher Q. Lan
Received: 3 February 2015 / Revised: 9 March 2015 / Accepted: 19 March 2015 / Published: 27 March 2015
(This article belongs to the Special Issue Microalgal Biotechnology)
View Full-Text   |   Download PDF [727 KB, uploaded 30 March 2015]   |  

Abstract

The key properties of microalgal biodiesel are largely determined by the composition of its fatty acid methyl esters (FAMEs). The gas chromatography (GC) based techniques for fatty acid analysis involve energy-intensive and time-consuming procedures and thus are less suitable for high-throughput screening applications. In the present study, a novel quantification method for microalgal fatty acids was established based on the near-infrared spectroscopy (NIRS) technique. The lyophilized cells of oleaginous Chlorella containing different contents of lipids were scanned by NIRS and their fatty acid profiles were determined by GC-MS. NIRS models were developed based on the chemometric correlation of the near-infrared spectra with fatty acid profiles in algal biomass. The optimized NIRS models showed excellent performances for predicting the contents of total fatty acids, C16:0, C18:0, C18:1 and C18:3, with the coefficient of determination (R2) being 0.998, 0.997, 0.989, 0.991 and 0.997, respectively. Taken together, the NIRS method established here bypasses the procedures of cell disruption, oil extraction and transesterification, is rapid, reliable, and of great potential for high-throughput applications, and will facilitate the screening of microalgal mutants and optimization of their growth conditions for biodiesel production. View Full-Text
Keywords: biodiesel; Chlorella; fatty acids; microalgae; near-infrared spectroscopy biodiesel; Chlorella; fatty acids; microalgae; near-infrared spectroscopy
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Liu, B.; Liu, J.; Chen, T.; Yang, B.; Jiang, Y.; Wei, D.; Chen, F. Rapid Characterization of Fatty Acids in Oleaginous Microalgae by Near-Infrared Spectroscopy. Int. J. Mol. Sci. 2015, 16, 7045-7056.

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