Next Article in Journal
High Insulin Levels in KK-Ay Diabetic Mice Cause Increased Cortical Bone Mass and Impaired Trabecular Micro-Structure
Next Article in Special Issue
Periodic CO2 Dosing Strategy for Dunaliella salina Batch Culture
Previous Article in Journal
Surface Modification of ZnO Nanorods with Hamilton Receptors
Previous Article in Special Issue
Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans
Article Menu
Issue 4 (April) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(4), 8201-8212; doi:10.3390/ijms16048201

Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma

1,2
,
2,3,†
,
2,†
,
1,2
,
4
,
1
and
2,5,*
1
School of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China
2
Institute for Food and Bioresource Engineering, College of Engineering, Peking University, Beijing 100871, China
3
College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China
4
School of Food Science, Jiangnan University, Wuxi 214122, China
5
Singapore-Peking University Research Centre for a Sustainable Low-Carbon Future, CREATE Tower 138602, Singapore
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Christopher Q. Lan
Received: 9 March 2015 / Revised: 1 April 2015 / Accepted: 7 April 2015 / Published: 13 April 2015
(This article belongs to the Special Issue Microalgal Biotechnology)
View Full-Text   |   Download PDF [757 KB, uploaded 13 April 2015]   |  

Abstract

Extracellular polysaccharides (EPS) produced by marine microalgae have the potential to be used as antioxidants, antiviral agents, immunomodulators, and anti-inflammatory agents. Although the marine microalga Crypthecodinium cohnii releases EPS during the process of docosahexaenoic acid (DHA) production, the yield of EPS remains relatively low. To improve the EPS production, a novel mutagenesis of C. cohnii was conducted by atmospheric and room temperature plasma (ARTP). Of the 12 mutants obtained, 10 mutants exhibited significantly enhanced EPS yield on biomass as compared with the wild type strain. Among them, mutant M7 was the best as it could produce an EPS volumetric yield of 1.02 g/L, EPS yield on biomass of 0.39 g/g and EPS yield on glucose of 94 mg/g, which were 33.85%, 85.35% and 57.17% higher than that of the wild type strain, respectively. Results of the present study indicated that mutagenesis of the marine microalga C. cohnii by ARTP was highly effective leading to the high-yield production of EPS. View Full-Text
Keywords: extracellular polysaccharides; atmospheric and room temperature plasma (ARTP); mutation; microalgae; Crypthecodinium cohnii extracellular polysaccharides; atmospheric and room temperature plasma (ARTP); mutation; microalgae; Crypthecodinium cohnii
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, B.; Sun, Z.; Ma, X.; Yang, B.; Jiang, Y.; Wei, D.; Chen, F. Mutation Breeding of Extracellular Polysaccharide-Producing Microalga Crypthecodinium cohnii by a Novel Mutagenesis with Atmospheric and Room Temperature Plasma. Int. J. Mol. Sci. 2015, 16, 8201-8212.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top