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Int. J. Mol. Sci. 2015, 16(4), 7551-7564; doi:10.3390/ijms16047551

In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes

1
Department of Biological Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea
2
Department of Chemical Engineering, Konkuk University, 120 Neungdong-ro, Gwangjin-Gu, Seoul 143701, Korea
3
Water Environment Center, Korea Institute of Science and Technology, P.O. Box 131, Cheongryang-Gu, Seoul 130650, Korea
4
Urban Agriculture Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Jeonju 560500, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 10 February 2015 / Revised: 19 March 2015 / Accepted: 30 March 2015 / Published: 3 April 2015
(This article belongs to the Collection Bioactive Nanoparticles)
View Full-Text   |   Download PDF [573 KB, uploaded 3 April 2015]   |  

Abstract

Water-dispersible MgO nanoparticles were tested to investigate their cytotoxic effects on oxidative stress gene expression. In this in vitro study, genes related to reactive oxygen species (ROS), glutathione S-transferase (GST) and catalase, were quantified using real-time polymerase chain reactions (molecular level) and molecular beacon technologies (cellular level). The monodispersed MgO nanoparticles, 20 nm in size, were used to treat human cancer cell lines (liver cancer epithelial cells) at different concentrations (25, 75 and 150 µg/mL) and incubation times (24, 48 and 72 h). Both the genetic and cellular cytotoxic screening methods produced consistent results, showing that GST and catalase ROS gene expression was maximized at 150 µg/mL nanoparticle treatment with 48 h incubation. However, the genotoxic effect of MgO nanoparticles was not significant compared with control experiments, which indicates its significant potential applications in nanomedicine as a diagnostic and therapeutic tool. View Full-Text
Keywords: catalase; nano-genotoxicity; MgO nanoparticle; glutathione S-transferase; reactive oxygen species catalase; nano-genotoxicity; MgO nanoparticle; glutathione S-transferase; reactive oxygen species
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kumaran, R.S.; Choi, Y.-K.; Singh, V.; Song, H.-J.; Song, K.-G.; Kim, K.J.; Kim, H.J. In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. Int. J. Mol. Sci. 2015, 16, 7551-7564.

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