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Int. J. Mol. Sci. 2015, 16(4), 7462-7477; doi:10.3390/ijms16047462

The Neuronal-Specific SGK1.1 (SGK1_v2) Kinase as a Transcriptional Modulator of BAG4, Brox, and PPP1CB Genes Expression

1
Laboratorio de Biología del Desarrollo, UD de Bioquímica y Biología Molecular and Centro de Investigaciones Biomédicas de Canarias (CIBICAN), Universidad de La Laguna, Av Astrofísico Sánchez s/n, La Laguna, Tenerife 38201, Spain
2
Department of Cellular and Molecular Physiology, Yale University, 333 Cedar Street, New Haven, CT 06510, USA
3
Department of Medicine A, Hematology, Hemostaseology, Oncology and Pneumology, University of Muenster, Muenster 48149, Germany
4
Department of Basic Medical Sciences, Tsinghua University School of Medicine, Beijing 100084, China
*
Author to whom correspondence should be addressed.
Academic Editor: Ali Mobasheri
Received: 30 January 2015 / Revised: 25 March 2015 / Accepted: 30 March 2015 / Published: 2 April 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [3050 KB, uploaded 2 April 2015]   |  

Abstract

The Serum- and Glucocorticoid-induced Kinase 1, SGK1, exhibits a broad range of cellular functions that include regulation of the number of ion channels in plasma membrane and modulation of signaling pathways of cell survival. This diversity of functions is made possible by various regulatory processes acting upon the SGK1 gene, giving rise to various isoforms: SGK1_v1–5, each with distinct properties and distinct aminotermini that serve to target proteins to different subcellular compartments. Among cellular effects of SGK1 expression is to indirectly modulate gene transcription by phosphorylating transcriptional factors of the FOXO family. Here we examined if SGK1.1 (SGK1_v2; NM_001143676), which associates primarily to the plasma membrane, is also able to regulate gene expression. Using a differential gene expression approach we identified six genes upregulated by SGK1.1 in HeLa cells. Further analysis of transcript and protein levels validated two genes: BCL2-associated athanogene 4 (BAG-4) and Brox. The results indicate that SGK1.1 regulates gene transcription upon a different set of genes some of which participate in cell survival pathways (BAG-4) and others in intracellular vesicular traffic (Brox). View Full-Text
Keywords: SGK1.1; transcription regulation; subtractive hybridization; transcriptional coactivator; Brox1 SGK1.1; transcription regulation; subtractive hybridization; transcriptional coactivator; Brox1
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

González-Fernández, R.; Ávila, J.; Arteaga, M.F.; Canessa, C.M.; Martín-Vasallo, P. The Neuronal-Specific SGK1.1 (SGK1_v2) Kinase as a Transcriptional Modulator of BAG4, Brox, and PPP1CB Genes Expression. Int. J. Mol. Sci. 2015, 16, 7462-7477.

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