Next Article in Journal
The Anti-TNF-α Antibody Infliximab Inhibits the Expression of Fat-Transporter-Protein FAT/CD36 in a Selective Hepatic-Radiation Mouse Model
Next Article in Special Issue
SLM Produced Porous Titanium Implant Improvements for Enhanced Vascularization and Osteoblast Seeding
Previous Article in Journal
Nerve Demyelination Increases Metabotropic Glutamate Receptor Subtype 5 Expression in Peripheral Painful Mononeuropathy
Previous Article in Special Issue
Accelerating in Situ Endothelialisation of Cardiovascular Bypass Grafts
Article Menu
Issue 3 (March) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2015, 16(3), 4666-4681; doi:10.3390/ijms16034666

A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue

Department of Biomedical Sciences, University of Padova, Viale Giuseppe Colombo, 3, 35131 Padova, Italy
Department of Neurosciences, University of Padova, Via Giustiniani, 2, 35131 Padova, Italy
Department of Stomatology and Biotechnologies, University of Chieti-Pescara, Via dei Vestini, 31, 66100 Chieti, Italy
These authors contributed equally to this work.
Author to whom correspondence should be addressed.
Academic Editors: Alexander M. Seifalian, Aaron Tan and Yasmin Farhatnia
Received: 22 December 2014 / Revised: 26 January 2015 / Accepted: 25 February 2015 / Published: 2 March 2015
(This article belongs to the Special Issue Artificial Organs)
View Full-Text   |   Download PDF [2391 KB, uploaded 2 March 2015]   |  


Dental pulp tissue supports the vitality of the tooth, but it is particularly vulnerable to external insults, such as mechanical trauma, chemical irritation or microbial invasion, which can lead to tissue necrosis. In the present work, we present an endodontic regeneration method based on the use of a tridimensional (3D) hyaluronan scaffold and human dental pulp stem cells (DPSCs) to produce a functional dental pulp-like tissue in vitro. An enriched population of DPSCs was seeded onto hyaluronan-based non-woven meshes in the presence of differentiation factors to induce the commitment of stem cells to neuronal, glial, endothelial and osteogenic phenotypes. In vitro experiments, among which were gene expression profiling and immunofluorescence (IF) staining, proved the commitment of DPSCs to the main components of dental pulp tissue. In particular, the hyaluronan-DPSCs construct showed a dental pulp-like morphology consisting of several specialized cells growing inside the hyaluronan fibers. Furthermore, these constructs were implanted into rat calvarial critical-size defects. Histological analyses and gene expression profiling performed on hyaluronan-DPSCs grafts showed the regeneration of osteodentin-like tissue. Altogether, these data suggest the regenerative potential of the hyaluronan-DPSC engineered tissue. View Full-Text
Keywords: hyaluronan; stem cells; human dental pulp stem cells (DPSCs); dental pulp; tissue engineering; regenerative endodontics hyaluronan; stem cells; human dental pulp stem cells (DPSCs); dental pulp; tissue engineering; regenerative endodontics

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Ferroni, L.; Gardin, C.; Sivolella, S.; Brunello, G.; Berengo, M.; Piattelli, A.; Bressan, E.; Zavan, B. A Hyaluronan-Based Scaffold for the in Vitro Construction of Dental Pulp-Like Tissue. Int. J. Mol. Sci. 2015, 16, 4666-4681.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top