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Int. J. Mol. Sci. 2015, 16(3), 4379-4391; doi:10.3390/ijms16034379

Hair-Growth-Promoting Effect of Conditioned Medium of High Integrin α6 and Low CD 71 (α6bri/CD71dim) Positive Keratinocyte Cells

Department of Dermatology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 138-736, Korea
Department of Applied Bioscience, CHA University, Seoul 135-081, Korea
Graduate School of New Drug Discovery and Development, Chungnam National University, Daejeon 305-764, Korea
Coway Cosmetics R&D Center, Seoul 153-792, Korea
Asan Institute for Life Sciences, Seoul 138-736, Korea
Department of Immunology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea
College of Pharmacy, Yonsei University, Incheon 406-840, Korea
Author to whom correspondence should be addressed.
Academic Editor: Bing Yan
Received: 22 October 2014 / Revised: 6 February 2015 / Accepted: 11 February 2015 / Published: 19 February 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [2778 KB, uploaded 19 February 2015]   |  


Keratinocyte stem/progenitor cells (KSCs) reside in the bulge region of the hair follicles and may be involved in hair growth. Hair follicle dermal papilla cells (HFDPCs) and outer root sheath (ORS) cells were treated with conditioned medium (CM) of KSCs. Moreover, the effects of KSC-CM on hair growth were examined ex vivo and in vivo. A human growth factor chip array and RT-PCR were employed to identify enriched proteins in KSC-CM as compared with CM from keratinocytes. KSC-CM significantly increased the proliferation of HFDPCs and ORS cells, and increased the S-phase of the cell cycle in HFDPCs. KSC-CM led to the phosphorylation of ATK and ERK1/2 in both cell types. After subcutaneous injection of KSC-CM in C3H/HeN mice, a significant increase in hair growth and increased proliferation of hair matrix keratinocytes ex vivo was observed. We identified six proteins enriched in KSC-CM (amphiregulin, insulin-like growth factor binding protein-2, insulin-like growth factor binding protein-5, granulocyte macrophage-colony stimulating factor, Platelet-derived growth factor-AA, and vascular endothelial growth factor). A growth-factor cocktail that contains these six recombinant growth factors significantly increased the proliferation of HFDPCs and ORS cells and enhanced the hair growth of mouse models. These results collectively indicate that KSC-CM has the potential to increase hair growth via the proliferative capacity of HFDPCs and ORS cells. View Full-Text
Keywords: conditioned media; hair growth; keratinocyte stem cells; paracrine effect conditioned media; hair growth; keratinocyte stem cells; paracrine effect

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Won, C.H.; Jeong, Y.-M.; Kang, S.; Koo, T.-S.; Park, S.-H.; Park, K.-Y.; Sung, Y.-K.; Sung, J.-H. Hair-Growth-Promoting Effect of Conditioned Medium of High Integrin α6 and Low CD 71 (α6bri/CD71dim) Positive Keratinocyte Cells. Int. J. Mol. Sci. 2015, 16, 4379-4391.

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