The Regulation and Function of miR-21-FOXO3a-miR-34b/c Signaling in Breast Cancer
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression of MiR-21 and MiR-34b/c in Breast Cancer Cell Lines, Clinical Specimens, and Serum Samples
2.2. Functions of MiR-21 and MiR-34b/c in Breast Cancer Cells
2.3. MiR-21 Regulates MiR-34b/c Expression via PTEN/AKT/FOXO3a Signaling Pathway
2.4. Systemic Delivery of MiR-34b/c Inhibits the Growth of Breast Tumors
2.5. Discussion
2.5.1. MiR-21 and MiR-34b/c Expression in Breast Cancer
2.5.2. Mechanism of MiR-21 Regulation of MiR-34b/c Expression
2.5.3. Functions of MiR-21 and MiR-34b/c in Breast Cancer
3. Experimental Section
3.1. Antibodies, Cell Lines, and Reagents
3.2. Patients and Tissues Samples
3.3. Quantitative RT-PCR
3.4. Cell Migration Assay
3.5. Immunohistochemistry Assay
3.6. Chromatin Immunoprecipitation-qPCR (ChIP-qPCR)
3.7. Mouse Xenograft Models
3.8. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Liu, X.; Feng, J.; Tang, L.; Liao, L.; Xu, Q.; Zhu, S. The Regulation and Function of miR-21-FOXO3a-miR-34b/c Signaling in Breast Cancer. Int. J. Mol. Sci. 2015, 16, 3148-3162. https://doi.org/10.3390/ijms16023148
Liu X, Feng J, Tang L, Liao L, Xu Q, Zhu S. The Regulation and Function of miR-21-FOXO3a-miR-34b/c Signaling in Breast Cancer. International Journal of Molecular Sciences. 2015; 16(2):3148-3162. https://doi.org/10.3390/ijms16023148
Chicago/Turabian StyleLiu, Xiangyan, Jie Feng, Lili Tang, Liqiu Liao, Qing Xu, and Shaihong Zhu. 2015. "The Regulation and Function of miR-21-FOXO3a-miR-34b/c Signaling in Breast Cancer" International Journal of Molecular Sciences 16, no. 2: 3148-3162. https://doi.org/10.3390/ijms16023148