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Int. J. Mol. Sci. 2015, 16(2), 2824-2838; doi:10.3390/ijms16022824

Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform

1
Institute for Synthetic Bioarchitectures, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria
2
Department of Biochemistry, King Abdulaziz University, Jeddah 21465, Saudi Arabia
3
Institute for Biophysics, Department of NanoBiotechnology, University of Natural Resources and Life Sciences, Muthgasse 11, Vienna 1190, Austria
Present address: Sysmex Austria GmbH, Odoakergasse 34-36, Vienna 1160, Austria
*
Author to whom correspondence should be addressed.
Academic Editor: Ritva Tikkanen
Received: 11 December 2014 / Revised: 8 January 2015 / Accepted: 22 January 2015 / Published: 27 January 2015
(This article belongs to the Special Issue Membrane Protein Based Biosensors)
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Abstract

The most important aspect of synthetic lipid membrane architectures is their ability to study functional membrane-active peptides and membrane proteins in an environment close to nature. Here, we report on the generation and performance of a biomimetic platform, the S-layer supported lipid membrane (SsLM), to investigate the structural and electrical characteristics of the membrane-active peptide gramicidin and the transmembrane protein α-hemolysin in real-time using a quartz crystal microbalance with dissipation monitoring in combination with electrochemical impedance spectroscopy. A shift in membrane resistance is caused by the interaction of α-hemolysin and gramicidin with SsLMs, even if only an attachment onto, or functional channels through the lipid membrane, respectively, are formed. Moreover, the obtained results did not indicate the formation of functional α-hemolysin pores, but evidence for functional incorporation of gramicidin into this biomimetic architecture is provided. View Full-Text
Keywords: biomimetics; supported lipid membrane; S-layer; planar membrane platform; membrane-active peptide and protein biomimetics; supported lipid membrane; S-layer; planar membrane platform; membrane-active peptide and protein
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Damiati, S.; Schrems, A.; Sinner, E.-K.; Sleytr, U.B.; Schuster, B. Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform. Int. J. Mol. Sci. 2015, 16, 2824-2838.

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