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Int. J. Mol. Sci. 2015, 16(10), 23382-23389; doi:10.3390/ijms161023382

Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma

1
Department of Urology, University Hospital Brno and Masaryk University Brno, Brno 62500, Czech Republic
2
Department of Urologic Oncology, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic
3
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Brno 65653, Czech Republic
4
Central European Institute of Technology, Masaryk University, Brno 62500, Czech Republic
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Martin Pichler
Received: 22 August 2015 / Revised: 11 September 2015 / Accepted: 23 September 2015 / Published: 29 September 2015
(This article belongs to the Collection Regulation by Non-Coding RNAs)
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Abstract

Serum microRNAs are emerging as a clinically useful tool for early and non-invasive detection of various cancer types including renal cell carcinoma (RCC). Based on our previous results, we performed the study to analyze circulating serum miR-378 and miR-210 in patients with various histological subtypes of RCC. RNA was purified from blood serum samples of 195 RCC patients and 100 healthy controls. The levels of miR-378 and miR-210 in serum were determined absolutely using quantitative real-time PCR. Pre- and postoperative levels of both microRNAs were compared in 20 RCC patients. Significantly increased serum levels of both miR-378 and miR-210 enabled to clearly distinguish RCC patients and healthy controls with 80% sensitivity and 78% specificity if analyzed in combination (p < 0.0001), and their levels significantly decreased in the time period of three months after radical nephrectomy (p < 0.0001). Increased level of miR-378 positively correlates with disease-free survival (p = 0.036) and clinical stage (p = 0.0476). The analysis of serum miR-378 and miR-210 proved their potential to serve as powerful non-invasive diagnostic and prognostic biomarkers in RCC. View Full-Text
Keywords: renal cell carcinoma; microRNA; blood serum; biomarker renal cell carcinoma; microRNA; blood serum; biomarker
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Fedorko, M.; Stanik, M.; Iliev, R.; Redova-Lojova, M.; Machackova, T.; Svoboda, M.; Pacik, D.; Dolezel, J.; Slaby, O. Combination of MiR-378 and MiR-210 Serum Levels Enables Sensitive Detection of Renal Cell Carcinoma. Int. J. Mol. Sci. 2015, 16, 23382-23389.

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