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Int. J. Mol. Sci. 2015, 16(1), 1821-1839; doi:10.3390/ijms16011821

Non-Classical Gluconeogenesis-Dependent Glucose Metabolism in Rhipicephalus microplus Embryonic Cell Line BME26

1
Laboratory of Chemistry and Function of Proteins and Peptides, Animal Experimentation Unit, UENF, Av. Alberto Lamego, 2000, Horto, CEP 28013-602 Campos dos Goytacazes, RJ, Brazil
2
Laboratory of Infectious Diseases, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18, Kita-ku Sapporo 060-0818, Japan
3
Laboratory of Biochemistry Hatisaburo Masuda, Institute of Medical Biochemistry, Federal University of Rio de Janeiro, NUPEM-UFRJ/Macaé, Campus Macaé, Avenida São José do Barreto, São José do Barreto, CEP 27965-045 Macaé, RJ, Brazil
4
Center of Biotechnology, Federal University of Rio Grande do Sul, C.P. 15005, Av. Bento Gonçalves 9500, Prédio 43421, Campos do Vale, CEP 91501-970 Porto Alegre, RS, Brazil
*
Author to whom correspondence should be addressed.
Academic Editor: Marcello Iriti
Received: 7 October 2014 / Accepted: 31 December 2014 / Published: 14 January 2015
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

In this work we evaluated several genes involved in gluconeogenesis, glycolysis and glycogen metabolism, the major pathways for carbohydrate catabolism and anabolism, in the BME26 Rhipicephalus microplus embryonic cell line. Genetic and catalytic control of the genes and enzymes associated with these pathways are modulated by alterations in energy resource availability (primarily glucose). BME26 cells in media were investigated using three different glucose concentrations, and changes in the transcription levels of target genes in response to carbohydrate utilization were assessed. The results indicate that several genes, such as glycogen synthase (GS), glycogen synthase kinase 3 (GSK3), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6 phosphatase (GP) displayed mutual regulation in response to glucose treatment. Surprisingly, the transcription of gluconeogenic enzymes was found to increase alongside that of glycolytic enzymes, especially pyruvate kinase, with high glucose treatment. In addition, RNAi data from this study revealed that the transcription of gluconeogenic genes in BME26 cells is controlled by GSK-3. Collectively, these results improve our understanding of how glucose metabolism is regulated at the genetic level in tick cells. View Full-Text
Keywords: metabolism; gluconeogenesis; glycolysis; tick; gene expression; glucose metabolism; gluconeogenesis; glycolysis; tick; gene expression; glucose
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

da Silva, R.M.; Della Noce, B.; Waltero, C.F.; Costa, E.P.; de Abreu, L.A.; Githaka, N.W.; Moraes, J.; Gomes, H.F.; Konnai, S.; da Silva Vaz, I., Jr.; Ohashi, K.; Logullo, C. Non-Classical Gluconeogenesis-Dependent Glucose Metabolism in Rhipicephalus microplus Embryonic Cell Line BME26. Int. J. Mol. Sci. 2015, 16, 1821-1839.

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