Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells
Abstract
:1. Introduction
2. Results and Discussion
2.1. Sterol Regulatory Element-Binding Protein 1 (SREBP1) Overexpression in Dairy Cow Mammary Epithelial Cells (DCMECs) Increases the Expression of Lipogenic Genes and Key Enzymes of Fatty Acid Synthesis as Well as Triglyceride Secretion
2.2. SREBP1 Gene Silencing Decreases the Expression of Lipogenic Genes and Key Enzymes of Fatty Acid Synthesis and Decreases Triglyceride Secretion in DCMECs
2.3. The Effect of Stearic Acid and Serum on SREBP1 and mTOR Expression in the Regulation of Milk Fat Synthesis
2.4. Discussion
3. Experimental Section
3.1. Experimental Materials
3.2. Primary DCMEC Culture and Treatment
3.3. Gene Silencing of SREBP1
3.4. pGCMV–IRES–EGFP–SREBP1 Construction and Transfection
3.5. BODIPY Staining of Lipid Droplets
3.6. Detection of Triglyceride Secretion
3.7. RNA Extraction and Real-Time Quantitative PCR
3.8. Immunofluorescence Assay
3.9. Western Blotting Analysis
3.10. Bioinformatics Analysis
3.11. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Li, N.; Zhao, F.; Wei, C.; Liang, M.; Zhang, N.; Wang, C.; Li, Q.-Z.; Gao, X.-J. Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells. Int. J. Mol. Sci. 2014, 15, 16998-17013. https://doi.org/10.3390/ijms150916998
Li N, Zhao F, Wei C, Liang M, Zhang N, Wang C, Li Q-Z, Gao X-J. Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells. International Journal of Molecular Sciences. 2014; 15(9):16998-17013. https://doi.org/10.3390/ijms150916998
Chicago/Turabian StyleLi, Nan, Feng Zhao, Chenjie Wei, Mengyao Liang, Na Zhang, Chunmei Wang, Qing-Zhang Li, and Xue-Jun Gao. 2014. "Function of SREBP1 in the Milk Fat Synthesis of Dairy Cow Mammary Epithelial Cells" International Journal of Molecular Sciences 15, no. 9: 16998-17013. https://doi.org/10.3390/ijms150916998