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Int. J. Mol. Sci. 2014, 15(9), 15244-15258; https://doi.org/10.3390/ijms150915244

Production of Structured Phosphatidylcholine with High Content of DHA/EPA by Immobilized Phospholipase A1-Catalyzed Transesterification

1
College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China
2
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, China
*
Authors to whom correspondence should be addressed.
Received: 23 June 2014 / Revised: 19 August 2014 / Accepted: 20 August 2014 / Published: 28 August 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

This paper presents the synthesis of structured phosphatidylcholine (PC) enriched with docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) by transesterification of DHA/EPA-rich ethyl esters with PC using immobilized phospholipsase A1 (PLA1) in solvent-free medium. Firstly, liquid PLA1 was immobilized on resin D380, and it was found that a pH of 5 and a support/PLA1 ratio (w/v) of 1:3 were the best conditions for the adsorption. Secondly, the immobilized PLA1 was used to catalyze transesterification of PC and DHA/EPA-rich ethyl esters. The maximal incorporation of DHA and EPA achieved was 30.7% for 24 h of reaction at 55 °C using a substrate mass ratio (PC/ethyl esters) of 1:6, an immobilized PLA1 loading of 15% and water dosage of 1.25%. Then the reaction mixture was analyzed by 31P nuclear magnetic resonance (NMR). The composition of reaction product included 16.5% PC, 26.3% 2-diacyl-sn-glycero-3-lysophosphatidylcholine (1-LPC), 31.4% 1-diacyl-sn-glycero-3-lysophosphatidylcholine (2-LPC), and 25.8% sn-glycerol-3-phosphatidylcholine (GPC). View Full-Text
Keywords: immobilized phospholipsase A1; structured phosphatidylcholine; docosahexaenoic; eicosapentaenoic immobilized phospholipsase A1; structured phosphatidylcholine; docosahexaenoic; eicosapentaenoic
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Li, X.; Chen, J.-F.; Yang, B.; Li, D.-M.; Wang, Y.-H.; Wang, W.-F. Production of Structured Phosphatidylcholine with High Content of DHA/EPA by Immobilized Phospholipase A1-Catalyzed Transesterification. Int. J. Mol. Sci. 2014, 15, 15244-15258.

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