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A View of Pre-mRNA Splicing from RNase R Resistant RNAs
AbstractDuring pre-mRNA splicing, exons in the primary transcript are precisely connected to generate an mRNA. Intron lariat RNAs are formed as by-products of this process. In addition, some exonic circular RNAs (circRNAs) may also result from exon skipping as by-products. Lariat RNAs and circRNAs are both RNase R resistant RNAs. RNase R is a strong 3' to 5' exoribonuclease, which efficiently degrades linear RNAs, such as mRNAs and rRNAs; therefore, the circular parts of lariat RNAs and the circRNAs can be segregated from eukaryotic total RNAs by their RNase R resistance. Thus, RNase R resistant RNAs could provide unexplored splicing information not available from mRNAs. Analyses of these RNAs identified repeating splicing phenomena, such as re-splicing of mature mRNAs and nested splicing. Moreover, circRNA might function as microRNA sponges. There is an enormous variety of endogenous circRNAs, which are generally synthesized in cells and tissues.
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Suzuki, H.; Tsukahara, T. A View of Pre-mRNA Splicing from RNase R Resistant RNAs. Int. J. Mol. Sci. 2014, 15, 9331-9342.View more citation formats
Suzuki H, Tsukahara T. A View of Pre-mRNA Splicing from RNase R Resistant RNAs. International Journal of Molecular Sciences. 2014; 15(6):9331-9342.Chicago/Turabian Style
Suzuki, Hitoshi; Tsukahara, Toshifumi. 2014. "A View of Pre-mRNA Splicing from RNase R Resistant RNAs." Int. J. Mol. Sci. 15, no. 6: 9331-9342.