Int. J. Mol. Sci. 2014, 15(6), 9331-9342; doi:10.3390/ijms15069331
Review

A View of Pre-mRNA Splicing from RNase R Resistant RNAs

1,2,* email and 2email
Received: 8 April 2014; in revised form: 8 May 2014 / Accepted: 16 May 2014 / Published: 26 May 2014
(This article belongs to the Special Issue Pre-mRNA Splicing)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: During pre-mRNA splicing, exons in the primary transcript are precisely connected to generate an mRNA. Intron lariat RNAs are formed as by-products of this process. In addition, some exonic circular RNAs (circRNAs) may also result from exon skipping as by-products. Lariat RNAs and circRNAs are both RNase R resistant RNAs. RNase R is a strong 3' to 5' exoribonuclease, which efficiently degrades linear RNAs, such as mRNAs and rRNAs; therefore, the circular parts of lariat RNAs and the circRNAs can be segregated from eukaryotic total RNAs by their RNase R resistance. Thus, RNase R resistant RNAs could provide unexplored splicing information not available from mRNAs. Analyses of these RNAs identified repeating splicing phenomena, such as re-splicing of mature mRNAs and nested splicing. Moreover, circRNA might function as microRNA sponges. There is an enormous variety of endogenous circRNAs, which are generally synthesized in cells and tissues.
Keywords: pre-mRNA splicing; RNase R; circular RNA; multiexon skipping
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MDPI and ACS Style

Suzuki, H.; Tsukahara, T. A View of Pre-mRNA Splicing from RNase R Resistant RNAs. Int. J. Mol. Sci. 2014, 15, 9331-9342.

AMA Style

Suzuki H, Tsukahara T. A View of Pre-mRNA Splicing from RNase R Resistant RNAs. International Journal of Molecular Sciences. 2014; 15(6):9331-9342.

Chicago/Turabian Style

Suzuki, Hitoshi; Tsukahara, Toshifumi. 2014. "A View of Pre-mRNA Splicing from RNase R Resistant RNAs." Int. J. Mol. Sci. 15, no. 6: 9331-9342.

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