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Int. J. Mol. Sci. 2014, 15(6), 9343-9359; doi:10.3390/ijms15069343
Article

Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens

1,2
, 1
, 1
, 1
 and 1,*
1 State Key Laboratory of Agribiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China 2 Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China
* Author to whom correspondence should be addressed.
Received: 29 March 2014 / Revised: 25 April 2014 / Accepted: 13 May 2014 / Published: 26 May 2014
(This article belongs to the Section Bioinorganic Chemistry)
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Abstract

The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens.
Keywords: Zea mays; RACK1; disease resistance Zea mays; RACK1; disease resistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Wang, B.; Yu, J.; Zhu, D.; Chang, Y.; Zhao, Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. Int. J. Mol. Sci. 2014, 15, 9343-9359.

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