Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2014, 15(6), 9343-9359; doi:10.3390/ijms15069343
Article

Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens

1,2
, 1
, 1
, 1
 and 1,*
Received: 29 March 2014; in revised form: 25 April 2014 / Accepted: 13 May 2014 / Published: 26 May 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [2028 KB, uploaded 19 June 2014]
Abstract: The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens.
Keywords: Zea mays; RACK1; disease resistance Zea mays; RACK1; disease resistance
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Wang, B.; Yu, J.; Zhu, D.; Chang, Y.; Zhao, Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. Int. J. Mol. Sci. 2014, 15, 9343-9359.

AMA Style

Wang B, Yu J, Zhu D, Chang Y, Zhao Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. International Journal of Molecular Sciences. 2014; 15(6):9343-9359.

Chicago/Turabian Style

Wang, Baosheng; Yu, Jingjuan; Zhu, Dengyun; Chang, Yujie; Zhao, Qian. 2014. "Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens." Int. J. Mol. Sci. 15, no. 6: 9343-9359.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert