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Int. J. Mol. Sci. 2014, 15(6), 9343-9359; doi:10.3390/ijms15069343

Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens

1
State Key Laboratory of Agribiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China
2
Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Hohhot 010031, China
*
Author to whom correspondence should be addressed.
Received: 29 March 2014 / Revised: 25 April 2014 / Accepted: 13 May 2014 / Published: 26 May 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The receptor for activated C kinase 1 (RACK1) belongs to a protein subfamily containing a tryptophan-aspartic acid-domain (WD) repeat structure. Compelling evidence indicates that RACK1 can interact with many signal molecules and affect different signal transduction pathways. In this study, we cloned a maize RACK1 gene (ZmRACK1) by RT-PCR. The amino acid sequence of ZmRACK1 had seven WD repeats in which there were typical GH (glycine-histidine) and WD dipeptides. Comparison with OsRACK1 from rice revealed 89% identity at the amino acid level. Expression pattern analysis by RT-PCR showed that ZmRACK1 was expressed in all analyzed tissues of maize and that its transcription in leaves was induced by abscisic acid and jasmonate at a high concentration. Overexpression of ZmRACK1 in maize led to a reduction in symptoms caused by Exserohilum turcicum (Pass.) on maize leaves. The expression levels of the pathogenesis-related protein genes, PR-1 and PR-5, increased 2.5–3 times in transgenic maize, and reactive oxygen species production was more active than in the wild-type. Yeast two-hybrid assays showed that ZmRACK1 could interact with RAC1, RAR1 and SGT1. This study and previous work leads us to believe that ZmRACK1 may form a complex with regulators of plant disease resistance to coordinate maize reactions to pathogens.
Keywords: Zea mays; RACK1; disease resistance Zea mays; RACK1; disease resistance
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wang, B.; Yu, J.; Zhu, D.; Chang, Y.; Zhao, Q. Maize ZmRACK1 Is Involved in the Plant Response to Fungal Phytopathogens. Int. J. Mol. Sci. 2014, 15, 9343-9359.

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