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Int. J. Mol. Sci. 2014, 15(4), 6772-6796; doi:10.3390/ijms15046772
Article

Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori

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Received: 19 February 2014; in revised form: 25 March 2014 / Accepted: 10 April 2014 / Published: 22 April 2014
(This article belongs to the Special Issue Pre-mRNA Splicing)
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Abstract: Sexual differentiation in Bombyx mori is controlled by sex-specific splicing of Bmdsx, which results in the omission of exons 3 and 4 in a male-specific manner. In B. mori, insulin-like growth factor II mRNA-binding protein (Imp) is a male-specific factor involved in male-specific splicing of Bmdsx. Male-specific Imp mRNA results from the male-specific inclusion of exon 8. To verify the link between histone methylation and alternative RNA processing in Imp, we examined the effects of RNAi-mediated knockdown of several histone methyltransferases on the sex-specific mRNA expression of Imp. As a result, male-specific expression of Imp mRNA was completely abolished when expression of the H3K79 methyltransferase DOT1L was repressed to <10% of that in control males. Chromatin immunoprecipitation-quantitative PCR analysis revealed a higher distribution of H3K79me2 in normal males than in normal females across Imp. RNA polymerase II (RNAP II) processivity assays indicated that RNAi knockdown of DOT1L in males caused a twofold decrease in RNAP II processivity compared to that in control males, with almost equivalent levels to those observed in normal females. Inhibition of RNAP II-mediated elongation in male cells repressed the male-specific splicing of Imp. Our data suggest the possibility that H3K79me2 accumulation along Imp is associated with the male-specific alternative processing of Imp mRNA that results from increased RNAP II processivity.
Keywords: alternative splicing; DOT1L; histone H3 methylation; sex determination alternative splicing; DOT1L; histone H3 methylation; sex determination
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Suzuki, M.G.; Ito, H.; Aoki, F. Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori. Int. J. Mol. Sci. 2014, 15, 6772-6796.

AMA Style

Suzuki MG, Ito H, Aoki F. Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori. International Journal of Molecular Sciences. 2014; 15(4):6772-6796.

Chicago/Turabian Style

Suzuki, Masataka G.; Ito, Haruka; Aoki, Fugaku. 2014. "Effects of RNAi-Mediated Knockdown of Histone Methyltransferases on the Sex-Specific mRNA Expression of Imp in the Silkworm Bombyx mori." Int. J. Mol. Sci. 15, no. 4: 6772-6796.


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