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Int. J. Mol. Sci. 2014, 15(6), 10821-10834; doi:10.3390/ijms150610821
Article

Continuous Flow Atmospheric Pressure Laser Desorption/Ionization Using a 6–7-µm-Band Mid-Infrared Tunable Laser for Biomolecular Mass Spectrometry

1
, 1,* , 2
, 2
, 3
 and 1,4,5
1 Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan 2 JEOL Ltd., 1156 Nakagamicho, Akishima, Tokyo 196-0022, Japan 3 Suntory Institute for Bioorganic Research, Suntory Foundation for Life Sciences, 1-1-1 Wakayamadai, Shimamotocho, Mishimagun, Osaka 618-0024, Japan 4 Graduate School of Frontier Biosciences, Osaka University, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan 5 The Center of Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
* Author to whom correspondence should be addressed.
Received: 20 March 2014 / Revised: 28 May 2014 / Accepted: 4 June 2014 / Published: 16 June 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
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Abstract

A continuous flow atmospheric pressure laser desorption/ionization technique using a porous stainless steel probe and a 6–7-µm-band mid-infrared tunable laser was developed. This ion source is capable of direct ionization from a continuous flow with a high temporal stability. The 6–7-µm wavelength region corresponds to the characteristic absorption bands of various molecular vibration modes, including O–H, C=O, CH3 and C–N bonds. Consequently, many organic compounds and solvents, including water, have characteristic absorption peaks in this region. This ion source requires no additional matrix, and utilizes water or acetonitrile as the solvent matrix at several absorption peak wavelengths (6.05 and 7.27 µm, respectively). The distribution of multiply-charged peptide ions is extremely sensitive to the temperature of the heated capillary, which is the inlet of the mass spectrometer. This ionization technique has potential for the interface of liquid chromatography/mass spectrometry (LC/MS).
Keywords: mass spectrometry; atmospheric pressure laser ionization; mid-infrared tunable laser; peptides mass spectrometry; atmospheric pressure laser ionization; mid-infrared tunable laser; peptides
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Hiraguchi, R.; Hazama, H.; Senoo, K.; Yahata, Y.; Masuda, K.; Awazu, K. Continuous Flow Atmospheric Pressure Laser Desorption/Ionization Using a 6–7-µm-Band Mid-Infrared Tunable Laser for Biomolecular Mass Spectrometry. Int. J. Mol. Sci. 2014, 15, 10821-10834.

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