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Int. J. Mol. Sci. 2014, 15(6), 10067-10082; doi:10.3390/ijms150610067
Article

Direct Analysis of hCGβcf Glycosylation in Normal and Aberrant Pregnancy by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

1,2,3,* , 4
 and 1,3,4
Received: 25 April 2014; in revised form: 20 May 2014 / Accepted: 21 May 2014 / Published: 5 June 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
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Abstract: The analysis of human chorionic gonadotropin (hCG) in clinical chemistry laboratories by specific immunoassay is well established. However, changes in glycosylation are not as easily assayed and yet alterations in hCG glycosylation is associated with abnormal pregnancy. hCGβ-core fragment (hCGβcf) was isolated from the urine of women, pregnant with normal, molar and hyperemesis gravidarum pregnancies. Each sample was subjected to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI TOF MS) analysis following dithiothreitol (DTT) reduction and fingerprint spectra of peptide hCGβ 6–40 were analyzed. Samples were variably glycosylated, where most structures were small, core and largely mono-antennary. Larger single bi-antennary and mixtures of larger mono-antennary and bi-antennary moieties were also observed in some samples. Larger glycoforms were more abundant in the abnormal pregnancies and tri-antennary carbohydrate moieties were only observed in the samples from molar and hyperemesis gravidarum pregnancies. Given that such spectral profiling differences may be characteristic, development of small sample preparation for mass spectral analysis of hCG may lead to a simpler and faster approach to glycostructural analysis and potentially a novel clinical diagnostic test.
Keywords: hCG; hCGβcf; MALDI TOF MS; pregnancy; hydatidiform mole; hyperemesis gravidarum; glycosylation hCG; hCGβcf; MALDI TOF MS; pregnancy; hydatidiform mole; hyperemesis gravidarum; glycosylation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Iles, R.K.; Cole, L.A.; Butler, S.A. Direct Analysis of hCGβcf Glycosylation in Normal and Aberrant Pregnancy by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Int. J. Mol. Sci. 2014, 15, 10067-10082.

AMA Style

Iles RK, Cole LA, Butler SA. Direct Analysis of hCGβcf Glycosylation in Normal and Aberrant Pregnancy by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. International Journal of Molecular Sciences. 2014; 15(6):10067-10082.

Chicago/Turabian Style

Iles, Ray K.; Cole, Laurence A.; Butler, Stephen A. 2014. "Direct Analysis of hCGβcf Glycosylation in Normal and Aberrant Pregnancy by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry." Int. J. Mol. Sci. 15, no. 6: 10067-10082.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert