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Int. J. Mol. Sci. 2014, 15(7), 11234-11244; doi:10.3390/ijms150711234
Article

Development of Laser Ionization Techniques for Evaluation of the Effect of Cancer Drugs Using Imaging Mass Spectrometry

1
, 1,* , 2
, 3
 and 1,4,5
1 Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan 2 Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan 3 Graduate School of Science and Engineering, Tokyo Metropolitan University, 1-1 Minamiosawa Hachioji, Tokyo 192-0397, Japan 4 Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan 5 The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan
* Author to whom correspondence should be addressed.
Received: 20 March 2014 / Revised: 2 June 2014 / Accepted: 9 June 2014 / Published: 25 June 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
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Abstract

Recently, combined therapy using chemotherapy and photodynamic therapy (PDT) has been proposed as a means of improving treatment outcomes. In order to evaluate the efficacy of combined therapy, it is necessary to determine the distribution of the anticancer drug and the photosensitizer. We investigated the use of imaging mass spectrometry (IMS) to simultaneously observe the distributions of an anticancer drug and photosensitizer administered to cancer cells. In particular, we sought to increase the sensitivity of detection of the anticancer drug docetaxel and the photosensitizer protoporphyrin IX (PpIX) by optimizing the ionization-assisting reagents. When we used a matrix consisting of equal weights of a zeolite (NaY5.6) and a conventional organic matrix (6-aza-2-thiothymine) in matrix-assisted laser desorption/ionization, the signal intensity of the sodium-adducted ion of docetaxel (administered at 100 μM) increased about 13-fold. Moreover, we detected docetaxel with the zeolite matrix using the droplet method, and detected PpIX by fluorescence and IMS with α-cyano-4-hydroxycinnamic acid (CHCA) using the spray method.
Keywords: imaging mass spectrometry; laser ionization; anticancer drug; drug resistance; zeolite matrix imaging mass spectrometry; laser ionization; anticancer drug; drug resistance; zeolite matrix
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Kannen, H.; Hazama, H.; Kaneda, Y.; Fujino, T.; Awazu, K. Development of Laser Ionization Techniques for Evaluation of the Effect of Cancer Drugs Using Imaging Mass Spectrometry. Int. J. Mol. Sci. 2014, 15, 11234-11244.

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