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Int. J. Mol. Sci. 2014, 15(5), 8360-8371; doi:10.3390/ijms15058360

In Vitro Antiprotozoal Activity of Abietane Diterpenoids Isolated from Plectranthus barbatus Andr.

1
Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia
2
Department of Pharmacognosy, College of Pharmacy, Salman Abdulaziz University, Alkharj 11942, Saudi Arabia
3
Laboratory for Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, Antwerp University, Universiteitsplein 1, B-2610 Antwerp, Belgium
*
Author to whom correspondence should be addressed.
Received: 11 February 2014 / Revised: 24 April 2014 / Accepted: 6 May 2014 / Published: 12 May 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Chromatographic separation of the n-hexane extract of the aerial part of Plectranthus barbatus led to the isolation of five abietane-type diterpenes: dehydroabietane (1); 5,6-didehydro-7-hydroxy-taxodone (2); taxodione (3); 20-deoxocarnosol (4) and 6α,11,12,-trihydroxy-7β,20-epoxy-8,11,13-abietatriene (5). The structures were determined using spectroscopic methods including one- and two-dimensional NMR methods. Compounds (1)–(3) and (5) are isolated here for the first time from the genus Plectranthus. The isolated abietane-type diterpenes tested in vitro for their antiprotozoal activity against erythrocytic schizonts of Plasmodium falciparum, intracellular amastigotes of Leishmania infantum and Trypanosoma cruzi and free trypomastigotes of T. brucei. Cytotoxicity was determined against fibroblast cell line MRC-5. Compound (2) 5,6-didehydro-7-hydroxy-taxodone showed remarkable activity with acceptable selectivity against P. falciparum (IC50 9.2 µM, SI 10.4) and T. brucei (IC50 1.9 µM, SI 50.5). Compounds (3)–(5) exhibited non-specific antiprotozoal activity due to high cytotoxicity. Compound (1) dehydroabietane showed no antiprotozoal potential. View Full-Text
Keywords: Plectranthus barbatus; antiprotozoal; abietane-type diterpenoids; Plasmodium; Leishmania; Trypanosoma; cytotoxicity Plectranthus barbatus; antiprotozoal; abietane-type diterpenoids; Plasmodium; Leishmania; Trypanosoma; cytotoxicity
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Mothana, R.A.; Al-Said, M.S.; Al-Musayeib, N.M.; Gamal, A.A.E.; Al-Massarani, S.M.; Al-Rehaily, A.J.; Abdulkader, M.; Maes, L. In Vitro Antiprotozoal Activity of Abietane Diterpenoids Isolated from Plectranthus barbatus Andr.. Int. J. Mol. Sci. 2014, 15, 8360-8371.

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