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Int. J. Mol. Sci. 2014, 15(4), 6674-6688; doi:10.3390/ijms15046674

Proteomic Analysis of Etiolated Juvenile Tetraploid Robinia pseudoacacia Branches during Different Cutting Periods

1,†
,
2,†
,
1,3,†
,
1
,
4
,
5
and
1,*
1
National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
2
Key Laboratory for Silviculture and Conservation, Ministry of Education, Beijing Forestry University, Beijing 100083, China
3
Beijing Municipal Bureau of Landscaping, Beijing 100029, China
4
Henan Academy of Forestry Science, Zhengzhou 450008, Henan, China
5
The State-Owned Luoning County Lv Cun Forest Farm, Luoning 471711, Henan, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 17 December 2013 / Revised: 31 March 2014 / Accepted: 2 April 2014 / Published: 21 April 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

The propagation of hard-branch cuttings of tetraploid Robinia pseudoacacia (black locust) is restricted by the low rooting rate; however, etiolated juvenile tetraploid black locust branches result in a significantly higher rooting rate of cuttings compared with non-etiolated juvenile tetraploid branches. To identify proteins that influence the juvenile tetraploid branch rooting process, two-dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectra (MALDI-TOF/TOF-MS) were used to analyze proteomic differences in the phloem of tetraploid R. pseudoacacia etiolated and non-etiolated juvenile branches during different cutting periods. A total of 58 protein spots differed in expression level, and 16 protein spots were only expressed in etiolated branches or non-etiolated ones. A total of 40 highly expressed protein spots were identified by mass spectrometry, 14 of which were accurately retrieved. They include nucleoglucoprotein metabolic proteins, signaling proteins, lignin synthesis proteins and phyllochlorin. These results help to reveal the mechanism of juvenile tetraploid R. pseudoacacia etiolated branch rooting and provide a valuable reference for the improvement of tetraploid R. pseudoacacia cutting techniques. View Full-Text
Keywords: tetraploid Robinia pseudoacacia; etiolated juvenile branch; cutting; proteomics tetraploid Robinia pseudoacacia; etiolated juvenile branch; cutting; proteomics
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MDPI and ACS Style

Lu, N.; Xu, Z.; Meng, B.; Sun, Y.; Zhang, J.; Wang, S.; Li, Y. Proteomic Analysis of Etiolated Juvenile Tetraploid Robinia pseudoacacia Branches during Different Cutting Periods. Int. J. Mol. Sci. 2014, 15, 6674-6688.

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