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Int. J. Mol. Sci. 2014, 15(4), 6689-6702; doi:10.3390/ijms15046689

Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase

1,† and 2,*
1 Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain 2 Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain 3 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia In memoriam.
* Author to whom correspondence should be addressed.
Received: 3 January 2014 / Revised: 3 March 2014 / Accepted: 9 April 2014 / Published: 21 April 2014
(This article belongs to the collection Advances in Proteomic Research)
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By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
Keywords: Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Aquilini, E.; Merino, S.; Knirel, Y.A.; Regué, M.; Tomás, J.M. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. Int. J. Mol. Sci. 2014, 15, 6689-6702.

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