Next Article in Journal
Polymorphisms in DNA Repair Genes and MDR1 and the Risk for Non-Hodgkin Lymphoma
Next Article in Special Issue
Biomarkers in Alzheimer’s Disease Analysis by Mass Spectrometry-Based Proteomics
Previous Article in Journal
Proteomic Analysis of Etiolated Juvenile Tetraploid Robinia pseudoacacia Branches during Different Cutting Periods
Previous Article in Special Issue
Quality Control and Stability Studies with the Monoclonal Antibody, Trastuzumab: Application of 1D- vs. 2D-Gel Electrophoresis
Int. J. Mol. Sci. 2014, 15(4), 6689-6702; doi:10.3390/ijms15046689

Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase

1,† and 2,*
1 Department Microbiology and Parasitology, Faculty Pharmacy, University of Barcelona, Av. Joan XXIII s/n, 08028 Barcelona, Spain 2 Department Microbiology, Faculty Biology, University Barcelona, Diagonal 643, 08071 Barcelona, Spain 3 N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow 119991, Russia In memoriam.
* Author to whom correspondence should be addressed.
Received: 3 January 2014 / Revised: 3 March 2014 / Accepted: 9 April 2014 / Published: 21 April 2014
(This article belongs to the collection Advances in Proteomic Research)
View Full-Text   |   Download PDF [1471 KB, uploaded 19 June 2014]   |   Browse Figures


By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
Keywords: Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
MDPI and ACS Style

Aquilini, E.; Merino, S.; Knirel, Y.A.; Regué, M.; Tomás, J.M. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. Int. J. Mol. Sci. 2014, 15, 6689-6702.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


Cited By

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert