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Int. J. Mol. Sci. 2014, 15(4), 6689-6702; doi:10.3390/ijms15046689
Article

Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase

1,2
, 2
, 3
, 1,† and 2,*
Received: 3 January 2014; in revised form: 3 March 2014 / Accepted: 9 April 2014 / Published: 21 April 2014
(This article belongs to the collection Advances in Proteomic Research)
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Abstract: By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
Keywords: Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine Proteus mirabilis; core lipopolysaccharide; phosphoethanolamine
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Aquilini, E.; Merino, S.; Knirel, Y.A.; Regué, M.; Tomás, J.M. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. Int. J. Mol. Sci. 2014, 15, 6689-6702.

AMA Style

Aquilini E, Merino S, Knirel YA, Regué M, Tomás JM. Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. International Journal of Molecular Sciences. 2014; 15(4):6689-6702.

Chicago/Turabian Style

Aquilini, Eleonora; Merino, Susana; Knirel, Yuriy A.; Regué, Miguel; Tomás, Juan M. 2014. "Functional Identification of Proteus mirabilis eptC Gene Encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase." Int. J. Mol. Sci. 15, no. 4: 6689-6702.


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