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Int. J. Mol. Sci. 2014, 15(4), 6657-6673; doi:10.3390/ijms15046657
Article

The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside

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Received: 24 January 2014; in revised form: 4 April 2014 / Accepted: 8 April 2014 / Published: 17 April 2014
(This article belongs to the collection Programmed Cell Death and Apoptosis)
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Abstract: Objective: Survivin is a member of the inhibitor of apoptosis protein family, which uniquely promotes mitosis and regulates apoptosis in cancer cells. Recent studies have demonstrated that survivin also expresses in several normal adult cells. In the present study, we aimed to investigate the function of survivin in the terminally differentiated epithelial cells, podocytes. Methods: Survivin expression and location were detected by Quantitative Real-Time PCR, western blot and fluorescence confocal microscopy methods in normal and injured mouse podocytes. Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin. Results: In podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement. Conclusion: Our data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. The mechanism of survivin related anti-apoptosis may, at least partially, be through the activation of caspase 3.
Keywords: survivin; podocyte apoptosis; nephropathy; cyto-protection survivin; podocyte apoptosis; nephropathy; cyto-protection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Li, X.; Zhang, X.; Li, X.; Ding, F.; Ding, J. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. Int. J. Mol. Sci. 2014, 15, 6657-6673.

AMA Style

Li X, Zhang X, Li X, Ding F, Ding J. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. International Journal of Molecular Sciences. 2014; 15(4):6657-6673.

Chicago/Turabian Style

Li, Xuejuan; Zhang, Xiaoyan; Li, Xiaoyan; Ding, Fangrui; Ding, Jie. 2014. "The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside." Int. J. Mol. Sci. 15, no. 4: 6657-6673.


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