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Int. J. Mol. Sci. 2014, 15(4), 6657-6673; doi:10.3390/ijms15046657

The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside

Department of Pediatrics, Peking University First Hospital, No.1 Xi An Men Da Jie, Beijing 100034, China
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Author to whom correspondence should be addressed.
Received: 24 January 2014 / Revised: 4 April 2014 / Accepted: 8 April 2014 / Published: 17 April 2014
(This article belongs to the Collection Programmed Cell Death and Apoptosis)
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Abstract

Objective: Survivin is a member of the inhibitor of apoptosis protein family, which uniquely promotes mitosis and regulates apoptosis in cancer cells. Recent studies have demonstrated that survivin also expresses in several normal adult cells. In the present study, we aimed to investigate the function of survivin in the terminally differentiated epithelial cells, podocytes. Methods: Survivin expression and location were detected by Quantitative Real-Time PCR, western blot and fluorescence confocal microscopy methods in normal and injured mouse podocytes. Cyto-protection function of survivin was also studied in cultured podocyte injured by puromycin aminonucleoside (PAN), transfected with survivin siRNA to down-regulate survivin expression, or with survivin plasmid to transiently over-express survivin. Results: In podocytes, PAN stimulated expressions of survivin and the apoptosis related molecule caspase 3. Knockdown of survivin expression by siRNA increased the activation of caspase 3, induced podocyte apoptosis and remarkable rearrangement of actin cytoskeleton. Moreover, over-expression of survivin inhibited PAN-induced podocyte apoptosis and cytoskeleton rearrangement. Conclusion: Our data provides the evidence that survivin plays an important role in protecting podocytes from apoptosis induced by PAN. The mechanism of survivin related anti-apoptosis may, at least partially, be through the activation of caspase 3. View Full-Text
Keywords: survivin; podocyte apoptosis; nephropathy; cyto-protection survivin; podocyte apoptosis; nephropathy; cyto-protection
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MDPI and ACS Style

Li, X.; Zhang, X.; Li, X.; Ding, F.; Ding, J. The Role of Survivin in Podocyte Injury Induced by Puromycin Aminonucleoside. Int. J. Mol. Sci. 2014, 15, 6657-6673.

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