Next Article in Journal
Triterpenoid Saponin Biosynthetic Pathway Profiling and Candidate Gene Mining of the Ilex asprella Root Using RNA-Seq
Next Article in Special Issue
Substance P Activates the Wnt Signal Transduction Pathway and Enhances the Differentiation of Mouse Preosteoblastic MC3T3-E1 Cells
Previous Article in Journal
Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats
Previous Article in Special Issue
Effects of Neuropeptides and Mechanical Loading on Bone Cell Resorption in Vitro
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(4), 5952-5969; doi:10.3390/ijms15045952

Leucyl-tRNA Synthetase Regulates Lactation and Cell Proliferation via mTOR Signaling in Dairy Cow Mammary Epithelial Cells

Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University, Harbin 150030, China
*
Author to whom correspondence should be addressed.
Received: 6 February 2014 / Revised: 28 March 2014 / Accepted: 28 March 2014 / Published: 9 April 2014
(This article belongs to the Special Issue Signalling Molecules and Signal Transduction in Cells 2014)
View Full-Text   |   Download PDF [686 KB, uploaded 19 June 2014]   |  

Abstract

The role of LeuRS, an aminoacyl-tRNA synthetase, as an intracellular l-leucine sensor for the mTORC1 pathway has been the subject of much research recently. Despite this, the association between LeuRS and lactation in dairy cow mammary epithelial cells (DCMECs) remains unknown. In this study, we found that LeuRS expression in mammary gland tissue was significantly higher during lactation than pregnancy. Moreover, our data demonstrates that LeuRS is localized in the cytoplasm. Treatment with leucine increased DCMECs viability and proliferation, as well as mammalian target of rapamycin (mTOR), p-mTOR, ribosomal protein S6 kinase 1 (S6K1), p-S6K1, β-Casein, sterol regulatory element binding protein 1c (SREBP-1c), glucose transporter 1 (GLUT1), and Cyclin D1 mRNA and protein expression. Secretion of lactose and triglyceride were also increased. siRNA-mediated knockdown of LeuRS led to reduction in all of these processes. Based on these data, LeuRS up-regulates the mTOR pathway to promote proliferation and lactation of DCMECs in response to changes in the intracellular leucine concentration.
Keywords: dairy cow mammary epithelial cells; lactation; leucine; cell proliferation; LeuRS; mTOR dairy cow mammary epithelial cells; lactation; leucine; cell proliferation; LeuRS; mTOR
Figures

This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Wang, L.; Lin, Y.; Bian, Y.; Liu, L.; Shao, L.; Lin, L.; Qu, B.; Zhao, F.; Gao, X.; Li, Q. Leucyl-tRNA Synthetase Regulates Lactation and Cell Proliferation via mTOR Signaling in Dairy Cow Mammary Epithelial Cells. Int. J. Mol. Sci. 2014, 15, 5952-5969.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top