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Int. J. Mol. Sci. 2014, 15(4), 5940-5951; doi:10.3390/ijms15045940
Article

Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats

1
, 2
 and 1,*
Received: 25 December 2013; in revised form: 24 March 2014 / Accepted: 31 March 2014 / Published: 9 April 2014
(This article belongs to the Special Issue Neuroprotective Strategies 2014)
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Abstract: Endogenous melatonin is a known free radical scavenger that removes reactive oxygen species (ROS), thus, alleviating oxidative stress. The purpose of this study was to demonstrate its effect against kainic acid (KA)-induced oxidative stress in organotypic hippocampal slice cultures (OHSCs). To observe neuroprotective effects of melatonin, different concentrations (0.01, 0.1 and 1 mM) of melatonin were administrated after KA treatment for 18 h in OHSCs of rat pups. Dose-response studies showed that neuronal cell death was significantly reduced after 0.1 and 1 mΜ melatonin treatments based on propidium iodide (PI) uptake and cresyl violet staining. The dichlorofluorescein (DCF) fluorescence which indicates ROS formation decreased more in the melatonin-treated group than in the KA group. The expression of 5-lipoxigenase (5-LO) and caspase-3 were reduced in the melatonin-treated groups compared to the KA group. These results suggest that melatonin may be an effective agent against KA-induced oxidative stress in the OHSC model.
Keywords: melatonin; kainic acid; organotypic hippocampal slice culture; reactive oxygen species; antioxidant; neuroprotection melatonin; kainic acid; organotypic hippocampal slice culture; reactive oxygen species; antioxidant; neuroprotection
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kim, H.A.; Lee, K.H.; Lee, B.H. Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats. Int. J. Mol. Sci. 2014, 15, 5940-5951.

AMA Style

Kim HA, Lee KH, Lee BH. Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats. International Journal of Molecular Sciences. 2014; 15(4):5940-5951.

Chicago/Turabian Style

Kim, Hyung A.; Lee, Kyung H.; Lee, Bae H. 2014. "Neuroprotective Effect of Melatonin against Kainic Acid-Induced Oxidative Injury in Hippocampal Slice Culture of Rats." Int. J. Mol. Sci. 15, no. 4: 5940-5951.


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