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Int. J. Mol. Sci. 2014, 15(4), 5970-5987; doi:10.3390/ijms15045970

Triterpenoid Saponin Biosynthetic Pathway Profiling and Candidate Gene Mining of the Ilex asprella Root Using RNA-Seq

Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
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Received: 26 January 2014 / Revised: 23 March 2014 / Accepted: 26 March 2014 / Published: 9 April 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Ilex asprella, which contains abundant α-amyrin type triterpenoid saponins, is an anti-influenza herbal drug widely used in south China. In this work, we first analysed the transcriptome of the I. asprella root using RNA-Seq, which provided a dataset for functional gene mining. mRNA was isolated from the total RNA of the I. asprella root and reverse-transcribed into cDNA. Then, the cDNA library was sequenced using an Illumina HiSeq™ 2000, which generated 55,028,452 clean reads. De novo assembly of these reads generated 51,865 unigenes, in which 39,269 unigenes were annotated (75.71% yield). According to the structures of the triterpenoid saponins of I. asprella, a putative biosynthetic pathway downstream of 2,3-oxidosqualene was proposed and candidate unigenes in the transcriptome data that were potentially involved in the pathway were screened using homology-based BLAST and phylogenetic analysis. Further amplification and functional analysis of these putative unigenes will provide insight into the biosynthesis of Ilex triterpenoid saponins. View Full-Text
Keywords: Ilex asprella; triterpenoid saponins; biosynthesis; α-amyrin; RNA-Seq Ilex asprella; triterpenoid saponins; biosynthesis; α-amyrin; RNA-Seq
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MDPI and ACS Style

Zheng, X.; Xu, H.; Ma, X.; Zhan, R.; Chen, W. Triterpenoid Saponin Biosynthetic Pathway Profiling and Candidate Gene Mining of the Ilex asprella Root Using RNA-Seq. Int. J. Mol. Sci. 2014, 15, 5970-5987.

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