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Int. J. Mol. Sci. 2014, 15(4), 5350-5365; doi:10.3390/ijms15045350
Article

Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds

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Received: 25 January 2014; in revised form: 10 March 2014 / Accepted: 13 March 2014 / Published: 27 March 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: Maize seed storage proteins are a major source of human and livestock consumption. However, these proteins have poor nutritional value, because they are deficient in lysine and tryptophan. Much research has been done to elevate the lysine content by reducing zein content or regulating the activities of key enzymes in lysine metabolism. Using the naturally lysine-rich protein genes, sb401 and SBgLR, from potato, we previously increased the lysine and protein contents of maize seeds. Here, we examined another natural lysine-rich protein gene, GhLRP, from cotton, which increased the lysine content of transgenic maize seeds at levels varying from 16.2% to 65.0% relative to the wild-type. The total protein content was not distinctly different, except in the six transgenic lines. The lipid and starch levels did not differ substantially in Gossypium hirsutum L. lysine-rich protein (GhLRP) transgenic kernels when compared to wild-type. The agronomic characteristics of all the transgenic maize were also normal. GhLRP is a high-lysine protein candidate gene for increasing the lysine content of maize. This study provided a valuable model system for improving maize lysine content.
Keywords: GhLRP; lysine-rich protein; lysine; transgenic maize GhLRP; lysine-rich protein; lysine; transgenic maize
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Yue, J.; Li, C.; Zhao, Q.; Zhu, D.; Yu, J. Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds. Int. J. Mol. Sci. 2014, 15, 5350-5365.

AMA Style

Yue J, Li C, Zhao Q, Zhu D, Yu J. Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds. International Journal of Molecular Sciences. 2014; 15(4):5350-5365.

Chicago/Turabian Style

Yue, Jing; Li, Cong; Zhao, Qian; Zhu, Dengyun; Yu, Jingjuan. 2014. "Seed-Specific Expression of a Lysine-Rich Protein Gene, GhLRP, from Cotton Significantly Increases the Lysine Content in Maize Seeds." Int. J. Mol. Sci. 15, no. 4: 5350-5365.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert