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Int. J. Mol. Sci. 2014, 15(3), 4464-4480; doi:10.3390/ijms15034464
Article

Effect of Nitrate on Nodule and Root Growth of Soybean (Glycine max (L.) Merr.)

1,†
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Received: 27 January 2014; in revised form: 4 March 2014 / Accepted: 5 March 2014 / Published: 13 March 2014
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Abstract: The application of combined nitrogen, especially nitrate, to soybean plants is known to strongly inhibit nodule formation, growth and nitrogen fixation. In the present study, we measured the effects of supplying 5 mM nitrate on the growth of nodules, primary root, and lateral roots under light at 28 °C or dark at 18 °C conditions. Photographs of the nodulated roots were periodically taken by a digital camera at 1-h intervals, and the size of the nodules was measured with newly developed computer software. Nodule growth was depressed approximately 7 h after the addition of nitrate under light conditions. The nodule growth rate under dark conditions was almost half that under light conditions, and nodule growth was further suppressed by the addition of 5 mM nitrate. Similar results were observed for the extending growth rate of the primary root as those for nodule growth supplied with 5 mM nitrate under light/dark conditions. In contrast, the growth of lateral roots was promoted by the addition of 5 mM nitrate. The 2D-PAGE profiles of nodule protein showed similar patterns between the 0 and 5 mM nitrate treatments, which suggested that metabolic integrity may be maintained with the 5 mM nitrate treatment. Further studies are required to confirm whether light or temperature condition may give the primary effect on the growth of nodules and roots.
Keywords: soybean; nitrate; nodule; primary root; lateral root; light and dark conditions; 2D-PAGE; computer software soybean; nitrate; nodule; primary root; lateral root; light and dark conditions; 2D-PAGE; computer software
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Saito, A.; Tanabata, S.; Tanabata, T.; Tajima, S.; Ueno, M.; Ishikawa, S.; Ohtake, N.; Sueyoshi, K.; Ohyama, T. Effect of Nitrate on Nodule and Root Growth of Soybean (Glycine max (L.) Merr.). Int. J. Mol. Sci. 2014, 15, 4464-4480.

AMA Style

Saito A, Tanabata S, Tanabata T, Tajima S, Ueno M, Ishikawa S, Ohtake N, Sueyoshi K, Ohyama T. Effect of Nitrate on Nodule and Root Growth of Soybean (Glycine max (L.) Merr.). International Journal of Molecular Sciences. 2014; 15(3):4464-4480.

Chicago/Turabian Style

Saito, Akinori; Tanabata, Sayuri; Tanabata, Takanari; Tajima, Seiya; Ueno, Manabu; Ishikawa, Shinji; Ohtake, Norikuni; Sueyoshi, Kuni; Ohyama, Takuji. 2014. "Effect of Nitrate on Nodule and Root Growth of Soybean (Glycine max (L.) Merr.)." Int. J. Mol. Sci. 15, no. 3: 4464-4480.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert