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Int. J. Mol. Sci. 2014, 15(3), 4481-4491; doi:10.3390/ijms15034481

Proteomic Analysis of the Follicular Fluid of Tianzhu White Yak during Diestrus

1
Agricultural College, Ningxia University, Yinchuan 750021, China
2
College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China
3
College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China
*
Authors to whom correspondence should be addressed.
Received: 22 January 2014 / Revised: 28 February 2014 / Accepted: 10 March 2014 / Published: 13 March 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

The aim of this study was to identify differentially expressed proteins in the follicular fluid of Tianzhu white yak during diestrus. Follicles obtained from female yak were divided into four groups according to their diameter: 0–2, 2–4, 4–6 mm, and greater than 6 mm. The follicular fluid was directly aspirated from the follicles and mixed according to follicular size, and two-dimensional gel electrophoresis was carried out on the crude follicular fluid samples. Thirty-four differentially expressed spots were generated from these four sizes of follicles. Fourteen of these spots were analyzed by MALDI-TOF/TOF-MS and identified as: AS3MT, VDP, ANKRD6, C10orf107 protein, MRP4, MAPKAP1, AGO3, profilin-β-actin, SPT2 homolog, AGP, AR, RNF20, obscurin-like-1, and one unnamed protein. These proteins were first reported in follicular fluid, in addition to VDP and AGP. Based on existing knowledge of their function and patterns of expression, we hypothesize that most of these differentially expressed proteins play a role in ovarian follicular growth and development, dominant follicle selection, or follicular atresia and development of oocytes; however, the function of the other differentially expressed proteins in reproduction remains ambiguous.
Keywords: Tianzhu white yak; follicular fluid; proteomic analysis; two-dimensional gel electrophoresis; diestrus Tianzhu white yak; follicular fluid; proteomic analysis; two-dimensional gel electrophoresis; diestrus
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Tao, J.; Zhao, G.; Zhao, X.; Li, F.; Wu, X.; Hu, J.; Zhang, Y. Proteomic Analysis of the Follicular Fluid of Tianzhu White Yak during Diestrus. Int. J. Mol. Sci. 2014, 15, 4481-4491.

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