Next Article in Journal
Next Article in Special Issue
Previous Article in Journal
Previous Article in Special Issue
Int. J. Mol. Sci. 2014, 15(3), 3660-3670; doi:10.3390/ijms15033660
Communication

A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis

1
, 2
, 2,3
, 4
, 4
, 1
, 4,*  and 2,4,*
Received: 27 January 2014; in revised form: 14 February 2014 / Accepted: 14 February 2014 / Published: 28 February 2014
View Full-Text   |   Download PDF [547 KB, uploaded 19 June 2014]
Abstract: Rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. These associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. Most of our knowledge on the metabolism and the physiology of the bacteria during symbiosis derives from studying roots nodules of terrestrial plants. Here we used a proteomics approach to investigate the bacterial physiology of photosynthetic Bradyrhizobium sp. ORS278 during the symbiotic process with the semi aquatical plant Aeschynomene indica that forms root and stem nodules. We analyzed the proteomes of bacteria extracted from each type of nodule. First, we analyzed the bacteroid proteome at two different time points and found only minor variation between the bacterial proteomes of 2-week- and 3-week-old nodules. High conservation of the bacteroid proteome was also found when comparing stem nodules and root nodules. Among the stem nodule specific proteins were those related to the phototrophic ability of Bradyrhizobium sp. ORS278. Furthermore, we compared our data with those obtained during an extensive genetic screen previously published. The symbiotic role of four candidate genes which corresponding proteins were found massively produced in the nodules but not identified during this screening was examined. Mutant analysis suggested that in addition to the EtfAB system, the fixA locus is required for symbiotic efficiency.
Keywords: proteome; photosynthetic Bradyrhizobium; symbiotic nitrogen fixation; stem nodulation; fixA proteome; photosynthetic Bradyrhizobium; symbiotic nitrogen fixation; stem nodulation; fixA
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Delmotte, N.; Mondy, S.; Alunni, B.; Fardoux, J.; Chaintreuil, C.; Vorholt, J.A.; Giraud, E.; Gourion, B. A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis. Int. J. Mol. Sci. 2014, 15, 3660-3670.

AMA Style

Delmotte N, Mondy S, Alunni B, Fardoux J, Chaintreuil C, Vorholt JA, Giraud E, Gourion B. A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis. International Journal of Molecular Sciences. 2014; 15(3):3660-3670.

Chicago/Turabian Style

Delmotte, Nathanael; Mondy, Samuel; Alunni, Benoit; Fardoux, Joel; Chaintreuil, Clémence; Vorholt, Julia A.; Giraud, Eric; Gourion, Benjamin. 2014. "A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis." Int. J. Mol. Sci. 15, no. 3: 3660-3670.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert