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Int. J. Mol. Sci. 2014, 15(2), 2906-2915; doi:10.3390/ijms15022906
Communication

Trans-Cinnamic Acid Increases Adiponectin and the Phosphorylation of AMP-Activated Protein Kinase through G-Protein-Coupled Receptor Signaling in 3T3-L1 Adipocytes

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Received: 27 November 2013; in revised form: 24 January 2014 / Accepted: 10 February 2014 / Published: 19 February 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract: Adiponectin and intracellular 5'adenosine monophosphate-activated protein kinase (AMPK) are important modulators of glucose and fat metabolism. Cinnamon exerts beneficial effects by improving insulin sensitivity and blood lipids, e.g., through increasing adiponectin concentrations and AMPK activation. The underlying mechanism is unknown. The Gi/Go-protein-coupled receptor (GPR) 109A stimulates adiponectin secretion after binding its ligand niacin. Trans-cinnamic acid (tCA), a compound of cinnamon is another ligand. We hypothesize whether AMPK activation and adiponectin secretion by tCA is transmitted by GPR signaling. Differentiated 3T3-L1 cells were incubated with pertussis toxin (PTX), an inhibitor of Gi/Go-protein-coupling, and treated with different tCA concentrations. Treatment with tCA increased adiponectin and the pAMPK/AMPK ratio (p ≤ 0.001). PTX incubation abolished the increased pAMPK/AMPK ratio and adiponectin secretion. The latter remained increased compared to controls (p ≤ 0.002). tCA treatment stimulated adiponectin secretion and AMPK activation; the inhibitory effect of PTX suggests GPR is involved in tCA stimulated signaling.
Keywords: trans-cinnamic acid; Adiponectin; 5'adenosine monophosphate-activated protein kinase; G-protein-coupled receptor 109A trans-cinnamic acid; Adiponectin; 5'adenosine monophosphate-activated protein kinase; G-protein-coupled receptor 109A
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Kopp, C.; Singh, S.P.; Regenhard, P.; Müller, U.; Sauerwein, H.; Mielenz, M. Trans-Cinnamic Acid Increases Adiponectin and the Phosphorylation of AMP-Activated Protein Kinase through G-Protein-Coupled Receptor Signaling in 3T3-L1 Adipocytes. Int. J. Mol. Sci. 2014, 15, 2906-2915.

AMA Style

Kopp C, Singh SP, Regenhard P, Müller U, Sauerwein H, Mielenz M. Trans-Cinnamic Acid Increases Adiponectin and the Phosphorylation of AMP-Activated Protein Kinase through G-Protein-Coupled Receptor Signaling in 3T3-L1 Adipocytes. International Journal of Molecular Sciences. 2014; 15(2):2906-2915.

Chicago/Turabian Style

Kopp, Christina; Singh, Shiva P.; Regenhard, Petra; Müller, Ute; Sauerwein, Helga; Mielenz, Manfred. 2014. "Trans-Cinnamic Acid Increases Adiponectin and the Phosphorylation of AMP-Activated Protein Kinase through G-Protein-Coupled Receptor Signaling in 3T3-L1 Adipocytes." Int. J. Mol. Sci. 15, no. 2: 2906-2915.


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