Abstract: The aim of this study was to prepare nanosized Tripterygium wilfordii multi-glycoside (GTW) powders by the supercritical antisolvent precipitation process (SAS), and to evaluate the anti-inflammatory effects. Ethanol was used as solvent and carbon dioxide was used as an antisolvent. The effects of process parameters such as precipitation pressure (15–35 MPa), precipitation temperature (45–65 °C), drug solution flow rates (3–7 mL/min) and drug concentrations (10–30 mg/mL) were investigated. The nanospheres obtained with mean diameters ranged from 77.5 to 131.8 nm. The processed and unprocessed GTW were characterized by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy and thermal gravimetric analysis. The present study was designed to investigate the beneficial effect of the GTW nanoparticles on adjuvant-induced arthritis in albino rats. The processed and unprocessed GTW were tested against Freund’s complete adjuvant-induced arthritis in rats. Blood samples were collected for the estimation of interleukins (IL-1α, IL-1β) and tumor necrosis factor-α (TNF-α). It was concluded that physicochemical properties and anti-inflammatory activity of GTW nanoparticles could be improved by physical modification, such as particle size reduction using supercritical antisolvent (SAS) process. Further, SAS process was a powerful methodology for improving the physicochemical properties and anti-inflammatory activity of GTW.
Keywords: multi-glycoside; nanoparticle; supercritical antisolvent; physicochemical property; Tripterygium wilfordii
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Chen, F.; Li, T.; Li, S.; Hou, K.; Liu, Z.; Li, L.; Cui, G.; Zu, Y.; Yang, L. Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process. Int. J. Mol. Sci. 2014, 15, 2695-2711.
Chen F, Li T, Li S, Hou K, Liu Z, Li L, Cui G, Zu Y, Yang L. Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process. International Journal of Molecular Sciences. 2014; 15(2):2695-2711.
Chen, Fengli; Li, Tong; Li, Shuangyang; Hou, Kexin; Liu, Zaizhi; Li, Lili; Cui, Guoqiang; Zu, Yuangang; Yang, Lei. 2014. "Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process." Int. J. Mol. Sci. 15, no. 2: 2695-2711.