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Int. J. Mol. Sci. 2014, 15(2), 2695-2711; doi:10.3390/ijms15022695
Article

Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process

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Received: 6 January 2014; in revised form: 25 January 2014 / Accepted: 10 February 2014 / Published: 17 February 2014
(This article belongs to the Special Issue Bioactive Nanoparticles 2014)
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Abstract: The aim of this study was to prepare nanosized Tripterygium wilfordii multi-glycoside (GTW) powders by the supercritical antisolvent precipitation process (SAS), and to evaluate the anti-inflammatory effects. Ethanol was used as solvent and carbon dioxide was used as an antisolvent. The effects of process parameters such as precipitation pressure (15–35 MPa), precipitation temperature (45–65 °C), drug solution flow rates (3–7 mL/min) and drug concentrations (10–30 mg/mL) were investigated. The nanospheres obtained with mean diameters ranged from 77.5 to 131.8 nm. The processed and unprocessed GTW were characterized by scanning electron microscopy, X-ray diffraction, Fourier-transform infrared spectroscopy and thermal gravimetric analysis. The present study was designed to investigate the beneficial effect of the GTW nanoparticles on adjuvant-induced arthritis in albino rats. The processed and unprocessed GTW were tested against Freund’s complete adjuvant-induced arthritis in rats. Blood samples were collected for the estimation of interleukins (IL-1α, IL-1β) and tumor necrosis factor-α (TNF-α). It was concluded that physicochemical properties and anti-inflammatory activity of GTW nanoparticles could be improved by physical modification, such as particle size reduction using supercritical antisolvent (SAS) process. Further, SAS process was a powerful methodology for improving the physicochemical properties and anti-inflammatory activity of GTW.
Keywords: multi-glycoside; nanoparticle; supercritical antisolvent; physicochemical property; Tripterygium wilfordii multi-glycoside; nanoparticle; supercritical antisolvent; physicochemical property; Tripterygium wilfordii
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Chen, F.; Li, T.; Li, S.; Hou, K.; Liu, Z.; Li, L.; Cui, G.; Zu, Y.; Yang, L. Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process. Int. J. Mol. Sci. 2014, 15, 2695-2711.

AMA Style

Chen F, Li T, Li S, Hou K, Liu Z, Li L, Cui G, Zu Y, Yang L. Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process. International Journal of Molecular Sciences. 2014; 15(2):2695-2711.

Chicago/Turabian Style

Chen, Fengli; Li, Tong; Li, Shuangyang; Hou, Kexin; Liu, Zaizhi; Li, Lili; Cui, Guoqiang; Zu, Yuangang; Yang, Lei. 2014. "Preparation and Characterization of Tripterygium wilfordii Multi-Glycoside Nanoparticle Using Supercritical Anti-Solvent Process." Int. J. Mol. Sci. 15, no. 2: 2695-2711.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert