Abstract: By means of plasma polymerization, positively charged, nanometre-thin coatings can be applied to implant surfaces. The aim of the present study was to quantify the adhesion of human bone cells in vitro and to evaluate the bone ongrowth in vivo, on titanium surfaces modified by plasma polymer coatings. Different implant surface configurations were examined: titanium alloy (Ti6Al4V) coated with plasma-polymerized allylamine (PPAAm) and plasma-polymerized ethylenediamine (PPEDA) versus uncoated. Shear stress on human osteoblast-like MG-63 cells was investigated in vitro using a spinning disc device. Furthermore, bone-to-implant contact (BIC) was evaluated in vivo. Custom-made conical titanium implants were inserted at the medial tibia of female Sprague-Dawley rats. After a follow-up of six weeks, the BIC was determined by means of histomorphometry. The quantification of cell adhesion showed a significantly higher shear stress for MG-63 cells on PPAAm and PPEDA compared to uncoated Ti6Al4V. Uncoated titanium alloyed implants showed the lowest BIC (40.4%). Implants with PPAAm coating revealed a clear but not significant increase of the BIC (58.5%) and implants with PPEDA a significantly increased BIC (63.7%). In conclusion, plasma polymer coatings demonstrate enhanced cell adhesion and bone ongrowth compared to uncoated titanium surfaces.
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Gabler, C.; Zietz, C.; Göhler, R.; Fritsche, A.; Lindner, T.; Haenle, M.; Finke, B.; Meichsner, J.; Lenz, S.; Frerich, B.; Lüthen, F.; Nebe, J.B.; Bader, R. Evaluation of Osseointegration of Titanium Alloyed Implants Modified by Plasma Polymerization. Int. J. Mol. Sci. 2014, 15, 2454-2464.
Gabler C, Zietz C, Göhler R, Fritsche A, Lindner T, Haenle M, Finke B, Meichsner J, Lenz S, Frerich B, Lüthen F, Nebe JB, Bader R. Evaluation of Osseointegration of Titanium Alloyed Implants Modified by Plasma Polymerization. International Journal of Molecular Sciences. 2014; 15(2):2454-2464.
Gabler, Carolin; Zietz, Carmen; Göhler, Rebecca; Fritsche, Andreas; Lindner, Tobias; Haenle, Maximilian; Finke, Birgit; Meichsner, Jürgen; Lenz, Solvig; Frerich, Bernhard; Lüthen, Frank; Nebe, J. B.; Bader, Rainer. 2014. "Evaluation of Osseointegration of Titanium Alloyed Implants Modified by Plasma Polymerization." Int. J. Mol. Sci. 15, no. 2: 2454-2464.