Int. J. Mol. Sci. 2014, 15(2), 2465-2474; doi:10.3390/ijms15022465
Communication

Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus

1email, 2email, 3email, 4email, 4email and 4,* email
Received: 31 December 2013; in revised form: 26 January 2014 / Accepted: 29 January 2014 / Published: 11 February 2014
(This article belongs to the Special Issue Mass Spectrometry Application in Biology)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Amino acid substitutions in influenza A virus are the main reasons for both antigenic shift and virulence change, which result from non-synonymous mutations in the viral genome. Nucleocapsid protein (NP), one of the major structural proteins of influenza virus, is responsible for regulation of viral RNA synthesis and replication. In this report we used LC-MS/MS to analyze tryptic digestion of nucleocapsid protein of influenza virus (A/Puerto Rico/8/1934 H1N1), which was isolated and purified by SDS poly-acrylamide gel electrophoresis. Thus, LC-MS/MS analyses, coupled with manual de novo sequencing, allowed the determination of three substituted amino acid residues R452K, T423A and N430T in two tryptic peptides. The obtained results provided experimental evidence that amino acid substitutions resulted from non-synonymous gene mutations could be directly characterized by mass spectrometry in proteins of RNA viruses such as influenza A virus.
Keywords: nucleocapsid protein; influenza virus A virus; amino acid substitution; mass spectrometry
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MDPI and ACS Style

Li, Z.; Sun, W.; Wu, D.; Gao, X.; Sun, N.; Liu, N. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus. Int. J. Mol. Sci. 2014, 15, 2465-2474.

AMA Style

Li Z, Sun W, Wu D, Gao X, Sun N, Liu N. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus. International Journal of Molecular Sciences. 2014; 15(2):2465-2474.

Chicago/Turabian Style

Li, Zijian; Sun, Wanchun; Wu, Donglin; Gao, Xiang; Sun, Ningning; Liu, Ning. 2014. "Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus." Int. J. Mol. Sci. 15, no. 2: 2465-2474.


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