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Int. J. Mol. Sci. 2014, 15(2), 2359-2367; doi:10.3390/ijms15022359

Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector

1
Laboratorio de Fisiología y Biología Molecular Vegetal, Instituto de Agroindustria, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile
2
Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile
3
Laboratorio de Fisiología Vegetal, Departamento de Botánica, Universidad de Concepción, Casilla 160-C, Correo 3, Concepción 3801061, Chile
4
Vicerrectoría de Investigación y Postgrado, Universidad de La Frontera, Casilla 54D, Temuco 4811230, Chile
*
Author to whom correspondence should be addressed.
Received: 13 December 2013 / Revised: 18 January 2014 / Accepted: 28 January 2014 / Published: 7 February 2014
(This article belongs to the collection Proteins and Protein-Ligand Interactions)
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Abstract

The current study isolated and characterized the Lip3F9 polypeptide sequence of Deschampsia antarctica Desv. (GeneBank Accession Number JX846628), which was found to be comprised of 291 base pairs and was, moreover, expressed in Pichia pastoris X-33 cells. The enzyme was secreted after 24 h of P. pastoris culture incubation and through induction with methanol. The expressed protein showed maximum lipase activity (35 U/L) with an optimal temperature of 37 °C. The lipase-expressed enzyme lost 50% of its specific activity at 25 °C, a behavior characteristic of a psychrotolerant enzyme. Recombinant enzyme activity was measured in the presence of ionic and non-ionic detergents, and a decrease in enzyme activity was detected for all concentrations of ionic and non-ionic detergents assessed.
Keywords: Deschampsia antarctica; lipase; Pichia pastoris; detergent tolerance Deschampsia antarctica; lipase; Pichia pastoris; detergent tolerance
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Rabert, C.; Gutiérrez-Moraga, A.; Navarrete-Gallegos, A.; Navarrete-Campos, D.; Bravo, L.A.; Gidekel, M. Expression of a Deschampsia antarctica Desv. Polypeptide with Lipase Activity in a Pichia pastoris Vector. Int. J. Mol. Sci. 2014, 15, 2359-2367.

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