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Int. J. Mol. Sci. 2014, 15(2), 2346-2358; doi:10.3390/ijms15022346

Molecular Cloning, Structural Analysis and Tissue Expression of Protein Phosphatase 3 Catalytic Subunit Alpha Isoform (PPP3CA) Gene in Tianfu Goat Muscle

1
Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, Sichuan, China
2
Mianyang Agriculture Bureau, Mianyang 621000, Sichuan, China
*
Author to whom correspondence should be addressed.
Received: 9 December 2013 / Revised: 14 January 2014 / Accepted: 17 January 2014 / Published: 7 February 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Calcineurin, a Ca2+/calmodulin-dependent protein phosphatase, plays a critical role in controlling skeletal muscle fiber type. However, little information is available concerning the expression of calcineurin in goat. Therefore, protein phosphatase 3 catalytic subunit alpha isoform (PPP3CA) gene, also called calcineurin Aα, was cloned and its expression characterized in Tianfu goat muscle. Real time quantitative polymerase chain reaction (RT-qPCR) analyses revealed that Tianfu goat PPP3CA was detected in cardiac muscle, biceps femoris muscle, abdominal muscle, longissimus dors muscle, and soleus muscle. High expression levels were found in biceps femoris muscle, longissimus muscle and abdominal muscle (p < 0.01), and low expression levels were seen in cardiac muscle and soleus muscle (p > 0.05). In addition, the spatial-temporal mRNA expression levels showed different variation trends in different muscles with the age of the goats. Western blotting further revealed that PPP3CA protein was expressed in the above-mentioned tissues, with the highest level in biceps femoris muscle, and the lowest level in soleus muscle. In this study, we isolated the full-length coding sequence of Tianfu goat PPP3CA gene, analyzed its structure, and investigated its expression in different muscle tissues from different age stages. These results provide a foundation for understanding the function of the PPP3CA gene in goats. View Full-Text
Keywords: PPP3CA; cDNA clone; sequence analysis; structure prediction; expression analysis PPP3CA; cDNA clone; sequence analysis; structure prediction; expression analysis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Wan, L.; Ma, J.; Xu, G.; Wang, D.; Wang, N. Molecular Cloning, Structural Analysis and Tissue Expression of Protein Phosphatase 3 Catalytic Subunit Alpha Isoform (PPP3CA) Gene in Tianfu Goat Muscle. Int. J. Mol. Sci. 2014, 15, 2346-2358.

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