Abstract: In this study, we evaluated the effect of different doses of polysaccharides extracted from Caripia montagnei mushroom at different intervals of treatment on colonic injury in the model of colitis induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS). The FT-IR analysis and NMR showed that the polysaccharides from this species of mushroom are composed of α- and β-glucans. The colonic damage was evaluated by macroscopic, histological, biochemical and immunologic analyses. The results showed the reduction of colonic lesions in all groups treated with the glucans. Such glucans significantly reduced the levels of IL-6 (50 and 75 mg/kg, p < 0.05), a major inflammatory cytokine. Biochemical analyses showed that the glucans from C. montagnei acted on reducing levels of alkaline phosphatase (75 mg/kg, p < 0.01) and myeloperoxidase (p < 0.001), a result confirmed by the reduction of cellular infiltration observed microscopically. The increase of catalase activity possibly indicates a protective effect of these glucans on colonic tissue, confirming their anti-inflammatory potential.
Keywords: colitis; polysaccharides; mushroom; anti-inflammatory; Caripia montagnei
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da S. Nascimento Santos, M.; de M. Magalhães, J.E.; Castro, L.S.E.P.W.; de Sousa Pinheiro, T.; Sabry, D.A.; Nobre, L.T.D.B.; Lima, J.P.M.S.; Baseia, I.G.; Leite, E.L. Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis. Int. J. Mol. Sci. 2014, 15, 2368-2385.
da S. Nascimento Santos M, de M. Magalhães JE, Castro LSEPW, de Sousa Pinheiro T, Sabry DA, Nobre LTDB, Lima JPMS, Baseia IG, Leite EL. Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis. International Journal of Molecular Sciences. 2014; 15(2):2368-2385.
da S. Nascimento Santos, Marilia; de M. Magalhães, Joedyson E.; Castro, Luiza S.E.P.W.; de Sousa Pinheiro, Thuane; Sabry, Diego A.; Nobre, Leonardo T.D.B.; Lima, João P.M.S.; Baseia, Iuri G.; Leite, Edda L. 2014. "Effect of Glucans from Caripia montagnei Mushroom on TNBS-Induced Colitis." Int. J. Mol. Sci. 15, no. 2: 2368-2385.