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Int. J. Mol. Sci. 2014, 15(2), 2327-2345; doi:10.3390/ijms15022327
Article

Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity

1,2
, 2
, 1
, 3
, 3
, 2
, 3
 and 2,4,*
1 Laboratory of Beneficial Microorganisms, Functional Food and Health (LMBAFS), Faculty of Natural Sciences and Life, University of Abdelhamid Ibn Badis, Mostaganem 27000, Algeria 2 Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universite Libre de Bruxelles, Brussels 1050, Belgium 3 Laboratory of Experimental Medicine, CHU Charleroi, A. Vésale Hospital, Universite Libre de Bruxelles, Montigny-le-Tilleul 6110, Belgium 4 Analytical Platform of the Faculty of Pharmacy, Universite Libre de Bruxelles, Brussels 1050, Belgium
* Author to whom correspondence should be addressed.
Received: 12 November 2013 / Revised: 18 January 2014 / Accepted: 23 January 2014 / Published: 7 February 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
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Abstract

Because propolis contains many types of antioxidant compounds such as polyphenols and flavonoids, it can be useful in preventing oxidative damages. Ethyl acetate extracts of propolis from several Algerian regions show high activity by scavenging free radicals, preventing lipid peroxidation and inhibiting myeloperoxidase (MPO). By fractioning and assaying ethyl acetate extracts, it was observed that both polyphenols and flavonoids contribute to these activities. A correlation was observed between the polyphenol content and the MPO inhibition. However, it seems that kaempferol, a flavonoid, contributes mainly to the MPO inhibition. This molecule is in a high amount in the ethyl acetate extract and demonstrates the best efficiency towards the enzyme with an inhibiting concentration at 50% of 4 ± 2 µM.
Keywords: propolis; oxidative stress; free radical scavenging; myeloperoxidase inhibition; LDL oxidation inhibition propolis; oxidative stress; free radical scavenging; myeloperoxidase inhibition; LDL oxidation inhibition
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Boufadi, Y.M.; Soubhye, J.; Riazi, A.; Rousseau, A.; Vanhaeverbeek, M.; Nève, J.; Boudjeltia, K.Z.; Van Antwerpen, P. Characterization and Antioxidant Properties of Six Algerian Propolis Extracts: Ethyl Acetate Extracts Inhibit Myeloperoxidase Activity. Int. J. Mol. Sci. 2014, 15, 2327-2345.

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