Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding
AbstractUniform labeling of proteins with fluorescent donor and acceptor dyes with an equimolar ratio is paramount for accurate determination of Förster resonance energy transfer (FRET) efficiencies. In practice, however, the labeled protein population contains donor-labeled molecules that have no corresponding acceptor. These FRET-inactive donors contaminate the donor fluorescence signal, which leads to underestimation of FRET efficiencies in conventional fluorescence intensity and lifetime-based FRET experiments. Such contamination is avoided if FRET efficiencies are extracted from the rise time of acceptor fluorescence upon donor excitation. The reciprocal value of the rise time of acceptor fluorescence is equal to the decay rate of the FRET-active donor fluorescence. Here, we have determined rise times of sensitized acceptor fluorescence to study the folding of double-labeled apoflavodoxin molecules and show that this approach tracks the characteristics of apoflavodoxinʼs complex folding pathway. View Full-Text
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Lindhoud, S.; Westphal, A.H.; van Mierlo, C.P.M.; Visser, A.J.W.G.; Borst, J.W. Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding. Int. J. Mol. Sci. 2014, 15, 23836-23850.
Lindhoud S, Westphal AH, van Mierlo CPM, Visser AJWG, Borst JW. Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding. International Journal of Molecular Sciences. 2014; 15(12):23836-23850.Chicago/Turabian Style
Lindhoud, Simon; Westphal, Adrie H.; van Mierlo, Carlo P.M.; Visser, Antonie J.W.G.; Borst, Jan W. 2014. "Rise-Time of FRET-Acceptor Fluorescence Tracks Protein Folding." Int. J. Mol. Sci. 15, no. 12: 23836-23850.