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Int. J. Mol. Sci. 2014, 15(12), 23851-23877; doi:10.3390/ijms151223851

Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis

1
Division of BioAnalytical Chemistry, AIMMS Research Group of BioMolecular Analysis, VU University Amsterdam, De Boelelaan 1083, 1081 HV Amsterdam, The Netherlands
2
Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering, Faculty of Biology, Environmental Sciences and Chemistry, University of Alcalá, Ctra. Madrid-Barcelona Km. 33.600, 28871 Alcalá de Henares, Madrid, Spain
*
Author to whom correspondence should be addressed.
Received: 21 October 2014 / Revised: 15 December 2014 / Accepted: 16 December 2014 / Published: 22 December 2014
(This article belongs to the Special Issue Detection and Safety Assessment of Genetically Modified Organisms)
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Abstract

Advances in biotechnology have increased the demand for suitable analytical techniques for the analysis of genetically modified organisms. Study of the substantial equivalence, discrimination between transgenic and non-transgenic cultivars, study of the unintended effects caused by a genetic modification or their response to diverse situations or stress conditions (e.g., environmental, climatic, infections) are some of the concerns that need to be addressed. Capillary electrophoresis (CE) is emerging as an alternative to conventional techniques for the study and characterization of genetically modified organisms. This article reviews the most recent applications of CE for the analysis and characterization of transgenic cultivars in the last five years. Different strategies have been described depending on the level analyzed (DNA, proteins or metabolites). Capillary gel electrophoresis (CGE) has shown to be particularly useful for the analysis of DNA fragments amplified by PCR. Metabolites and proteins have been mainly separated using capillary zone electrophoresis (CZE) using UV and MS detection. Electrophoretic chips have also proven their ability in the analysis of transgenic cultivars and a section describing the new applications is also included. View Full-Text
Keywords: capillary electrophoresis; microchip electrophoresis; transgenic cultivars; GMO; safety assessment; food capillary electrophoresis; microchip electrophoresis; transgenic cultivars; GMO; safety assessment; food
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Vega, E.D.; Marina, M.L. Characterization and Study of Transgenic Cultivars by Capillary and Microchip Electrophoresis. Int. J. Mol. Sci. 2014, 15, 23851-23877.

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