Next Article in Journal
RNA Recognition and Stress Granule Formation by TIA Proteins
Previous Article in Journal
OPN Induces FoxM1 Expression and Localization through ERK 1/2, AKT, and p38 Signaling Pathway in HEC-1A Cells
Article Menu
Issue 12 (December) cover image

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2014, 15(12), 23359-23376; doi:10.3390/ijms151223359

Evaluation of Posterolateral Lumbar Fusion in Sheep Using Mineral Scaffolds Seeded with Cultured Bone Marrow Cells

1
Laboratory of Bioengineering and Tissue Regeneration (LABRET), Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, University of Málaga, Campus de Teatinos, Málaga 29071, Spain
2
Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER–BBN), Madrid 28029, Spain
3
Department of Pathological Anatomy, Faculty of Medicine, University of Cádiz, Cádiz 11003, Spain
4
Department of Surgery, Obstetrics and Gynecology, Faculty of Medicine, University of Málaga, Campus de Teatinos, Málaga 29071, Spain
5
Department of Orthopedic Surgery and Traumatology, Hospital Costa del Sol, Marbella 29603, Spain
6
Catalonian Institute for Advanced Chemistry (IQAC–CSIC), Barcelona 08034, Spain
7
Andalusian Centre for Nanomedicine and Biotechnology (BIONAND), University of Málaga, Málaga 29590, Spain
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 28 February 2014 / Revised: 25 October 2014 / Accepted: 24 November 2014 / Published: 16 December 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
View Full-Text   |   Download PDF [5767 KB, uploaded 16 December 2014]   |  

Abstract

The objective of this study is to investigate the efficacy of hybrid constructs in comparison to bone grafts (autograft and allograft) for posterolateral lumbar fusion (PLF) in sheep, instrumented with transpedicular screws and bars. Hybrid constructs using cultured bone marrow (BM) mesenchymal stem cells (MSCs) have shown promising results in several bone healing models. In particular, hybrid constructs made by calcium phosphate-enriched cells have had similar fusion rates to bone autografts in posterolateral lumbar fusion in sheep. In our study, four experimental spinal fusions in two animal groups were compared in sheep: autograft and allograft (reference group), hydroxyapatite scaffold, and hydroxyapatite scaffold seeded with cultured and osteoinduced bone marrow MSCs (hybrid construct). During the last three days of culture, dexamethasone (dex) and beta-glycerophosphate (β-GP) were added to potentiate osteoinduction. The two experimental situations of each group were tested in the same spinal segment (L4–L5). Spinal fusion and bone formation were studied by clinical observation, X-ray, computed tomography (CT), histology, and histomorphometry. Lumbar fusion rates assessed by CT scan and histology were higher for autograft and allograft (70%) than for mineral scaffold alone (22%) and hybrid constructs (35%). The quantity of new bone formation was also higher for the reference group, quite similar in both (autograft and allograft). Although the hybrid scaffold group had a better fusion rate than the non-hybrid scaffold group, the histological analysis revealed no significant differences between them in terms of quantity of bone formation. The histology results suggested that mineral scaffolds were partly resorbed in an early phase, and included in callus tissues. Far from the callus area the hydroxyapatite alone did not generate bone around it, but the hybrid scaffold did. In nude mice, labeled cells were induced to differentiate in vivo and monitored by bioluminescence imaging (BLI). Although the cultured MSCs had osteogenic potential, their contribution to spinal fusion when seeded in mineral scaffolds, in the conditions disclosed here, remains uncertain probably due to callus interference with the scaffolds. At present, bone autografts are better than hybrid constructs for posterolateral lumbar fusion, but we should continue to seek better conditions for efficient tissue engineering. View Full-Text
Keywords: spinal fusion; autograft; allograft; mesenchymal stem cell; scaffold; hydroxyapatite; tissue engineering; callus; CT scan; histology; histomorphometry spinal fusion; autograft; allograft; mesenchymal stem cell; scaffold; hydroxyapatite; tissue engineering; callus; CT scan; histology; histomorphometry
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Cuenca-López, M.D.; Andrades, J.A.; Gómez, S.; Zamora-Navas, P.; Guerado, E.; Rubio, N.; Blanco, J.; Becerra, J. Evaluation of Posterolateral Lumbar Fusion in Sheep Using Mineral Scaffolds Seeded with Cultured Bone Marrow Cells. Int. J. Mol. Sci. 2014, 15, 23359-23376.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top