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Int. J. Mol. Sci. 2014, 15(12), 21992-22010; doi:10.3390/ijms151221992

Identification of a Plastid-Localized Bifunctional Nerolidol/Linalool Synthase in Relation to Linalool Biosynthesis in Young Grape Berries

1
Centre for Viticulture & Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
2
Department of Food Science and Engineering, College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
3
Qingdao Huadong Winery Co., Ltd., Northside of Lisha Road, Laoshan District, Qingdao 266000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 2 September 2014 / Revised: 13 October 2014 / Accepted: 29 October 2014 / Published: 1 December 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Monoterpenoids are a diverse class of natural products and contribute to the important varietal aroma of certain Vitis vinifera grape cultivars. Among the typical monoterpenoids, linalool exists in almost all grape varieties. A gene coding for a nerolidol/linalool (NES/LINS) synthase was evaluated in the role of linalool biosynthesis in grape berries. Enzyme activity assay of this recombinant protein revealed that it could convert geranyl diphosphate and farnesyl diphosphate into linalool and nerolidol in vitro, respectively, and thus it was named VvRILinNer. However, localization experiment showed that this enzyme was only localized to chloroplasts, which indicates that VvRILinNer functions in the linalool production in vivo. The patterns of gene expression and linalool accumulation were analyzed in the berries of three grape cultivars (“Riesling”, “Cabernet Sauvignon”, “Gewurztraminer”) with significantly different levels of monoterpenoids. The VvRILinNer was considered to be mainly responsible for the synthesis of linalool at the early developmental stage. This finding has provided us with new knowledge to uncover the complex monoterpene biosynthesis in grapes. View Full-Text
Keywords: grape berries; linalool synthase; subcellular localization; gene expression grape berries; linalool synthase; subcellular localization; gene expression
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Zhu, B.-Q.; Cai, J.; Wang, Z.-Q.; Xu, X.-Q.; Duan, C.-Q.; Pan, Q.-H. Identification of a Plastid-Localized Bifunctional Nerolidol/Linalool Synthase in Relation to Linalool Biosynthesis in Young Grape Berries. Int. J. Mol. Sci. 2014, 15, 21992-22010.

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