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Int. J. Mol. Sci. 2014, 15(11), 21105-21119; doi:10.3390/ijms151121105

Growth Suppression of Colorectal Cancer by Plant-Derived Multiple mAb CO17-1A × BR55 via Inhibition of ERK1/2 Phosphorylation

1
Institute of Glycoscience, Wonkwang University, Iksan, Jeonbuk 570-749, Korea
2
Department of Biological Science, College of Natural Sciences, Wonkwang University, Iksan, Jeonbuk 570-749, Korea
3
Department of Medicine, Medical Research Institute, College of Medicine Chung-Ang University, Heukseok-ro 84, Seoul 156-756, Korea
4
Department of Agrofood Resources, National Academy of Agricultural Science, RDA, Suwon 441-853, Korea
5
Department of Clinical Laboratory Science, Wonkwang Health Science University, Iksan 570-750, Korea
*
Author to whom correspondence should be addressed.
Received: 16 September 2014 / Revised: 31 October 2014 / Accepted: 6 November 2014 / Published: 14 November 2014
(This article belongs to the Special Issue Advances in Molecular Oncology 2014)
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Abstract

We have generated the transgenic Tabaco plants expressing multiple monoclonal antibody (mAb) CO7-1A × BR55 by cross-pollinating with mAb CO17-1A and mAb BR55. We have demonstrated the anti-cancer effect of plant-derived multiple mAb CO17-1A × BR55. We find that co-treatment of colorectal mAbs (anti-epithelial cellular adhesion molecule (EpCAM), plant-derived monoclonal antibody (mAbP) CO17-1A and mAbP CO17-1A × BR55) with RAW264.7 cells significantly inhibited the cell growth in SW620 cancer cells. In particular, multi mAbP CO17-1A × BR55 significantly and efficiently suppressed the growth of SW620 cancer cells compared to another mAbs. Apoptotic death-positive cells were significantly increased in the mAbP CO17-1A × BR55-treated. The mAbP CO17-1A × BR55 treatment significantly decreased the expression of B-Cell lymphoma-2 (BCl-2), but the expression of Bcl-2-associated X protein (Bax), and cleaved caspase-3 were markedly increased. In vivo, the mAbP CO17-1A × BR55 significantly and efficiently inhibited the growth of colon tumors compared to another mAbs. The apoptotic cell death and inhibition of pro-apoptotic proteins expression were highest by treatment with mAbP CO17-1A × BR55. In addition, the mAbP CO17-1A × BR55 significantly inhibited the extracellular signal-regulated kinase 1 and 2 (ERK1/2) phosphorylation in cancer cells and tumors. Therefore, this study results suggest that multiple mAbP CO17-1A × BR55 has a significant effect on apoptosis-mediated anticancer by suppression of ERK1/2 phosphorylation in colon cancer compared to another mAbs. In light of these results, further clinical investigation should be conducted on mAbP CO17-1A × BR55 to determine its possible chemopreventive and/or therapeutic efficacy against human colon cancer. View Full-Text
Keywords: anti-EpCAM, colon cancer; mAbP CO17-1A × BR5; apoptosis; mAbP CO17-1A; monoclonal antibody anti-EpCAM, colon cancer; mAbP CO17-1A × BR5; apoptosis; mAbP CO17-1A; monoclonal antibody
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Kwak, D.H.; Moussavou, G.; Lee, J.H.; Heo, S.Y.; Ko, K.; Hwang, K.-A.; Jekal, S.-J.; Choo, Y.-K. Growth Suppression of Colorectal Cancer by Plant-Derived Multiple mAb CO17-1A × BR55 via Inhibition of ERK1/2 Phosphorylation. Int. J. Mol. Sci. 2014, 15, 21105-21119.

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