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Int. J. Mol. Sci. 2014, 15(11), 19741-19759; doi:10.3390/ijms151119741

Characterization of CaHsp70-1, a Pepper Heat-Shock Protein Gene in Response to Heat Stress and Some Regulation Exogenous Substances in Capsicum annuum L.

1
College of Horticulture, Northwest A&F University, Yangling 712100, China
2
Institute of Vegetables, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China
*
Authors to whom correspondence should be addressed.
Received: 27 September 2014 / Revised: 16 October 2014 / Accepted: 23 October 2014 / Published: 29 October 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Pepper (Capsicum annuum L.) is sensitive to heat stress (HS). Heat shock proteins 70 (Hsp70s) play a crucial role in protecting plant cells against HS and control varies characters in different plants. However, CaHsp70-1 gene was not well characterized in pepper. In this study, CaHsp70-1 was cloned from the pepper thermotolerant line R9, which encoded a protein of 652 amino acids, with a molecular weight of 71.54 kDa and an isoelectric point of 5.20. CaHsp70-1 belongs to the cytosolic Hsp70 subgroup, and best matched with tomato SlHsp70. CaHsp70-1 was highly induced in root, stem, leaf and flower in R9 with HS treatment (40 °C for 2 h). In both thermosensitive line B6 and thermotolerant line R9, CaHsp70-1 significantly increased after 0.5 h of HS (40 °C), and maintained in a higher level after 4 h HS. The expression of CaHsp70-1 induced by CaCl2, H2O2 and putrescine (Put) under HS were difference between B6 and R9 lines. The different expression patterns may be related to the differences in promoters of CaHsp70-1 from the two lines. These results suggest that CaHsp70-1 as a member of cytosolic Hsp70 subgroup, may be involved in HS defense response via a signal transduction pathway contained Ca2+, H2O2 and Put. View Full-Text
Keywords: Capsicum annuum L.; CaHsp70-1; heat stress; gene expression Capsicum annuum L.; CaHsp70-1; heat stress; gene expression
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Guo, M.; Zhai, Y.-F.; Lu, J.-P.; Chai, L.; Chai, W.-G.; Gong, Z.-H.; Lu, M.-H. Characterization of CaHsp70-1, a Pepper Heat-Shock Protein Gene in Response to Heat Stress and Some Regulation Exogenous Substances in Capsicum annuum L.. Int. J. Mol. Sci. 2014, 15, 19741-19759.

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