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Int. J. Mol. Sci. 2014, 15(10), 17667-17685; doi:10.3390/ijms151017667

De Novo Assembly and Characterization of Pericarp Transcriptome and Identification of Candidate Genes Mediating Fruit Cracking in Litchi chinensis Sonn.

1
Key Laboratory of Tropical Fruit Biology (Ministry of Agriculture), South Subtropical Crops Research Institute, Chinese Academy of Tropical Agricultural Sciences, Zhanjiang 524091, China
2
Basic Education College of Zhanjiang Normal University, Zhanjiang 524037, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 30 May 2014 / Revised: 12 September 2014 / Accepted: 19 September 2014 / Published: 30 September 2014
(This article belongs to the Section Biochemistry, Molecular and Cellular Biology)
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Abstract

Fruit cracking has long been a topic of great concern for growers and researchers of litchi (Litchi chinensis Sonn.). To understand the molecular mechanisms underlying fruit cracking, high-throughput RNA sequencing (RNA-Seq) was first used for de novo assembly and characterization of the transcriptome of cracking pericarp of litchi. Comparative transcriptomic analyses were performed on non-cracking and cracking fruits. A total of approximately 26 million and 29 million high quality reads were obtained from the two groups of samples, and were assembled into 46,641 unigenes with an average length of 993 bp. These unigenes can be useful resources for future molecular studies of the pericarp in litchi. Furthermore, four genes (LcAQP, 1; LcPIP, 1; LcNIP, 1; LcSIP, 1) involved in water transport, five genes (LcKS, 2; LcGA2ox, 2; LcGID1, 1) involved in GA metabolism, 21 genes (LcCYP707A, 2; LcGT, 9; Lcβ-Glu, 6; LcPP2C, 2; LcABI1, 1; LcABI5, 1) involved in ABA metabolism, 13 genes (LcTPC, 1; Ca2+/H+ exchanger, 3; Ca2+-ATPase, 4; LcCDPK, 2; LcCBL, 3) involved in Ca transport and 24 genes (LcPG, 5; LcEG, 1; LcPE, 3; LcEXP, 5; Lcβ-Gal, 9; LcXET, 1) involved in cell wall metabolism were identified as genes that are differentially expressed in cracked fruits compared to non-cracked fruits. Our results open new doors to further understand the molecular mechanisms behind fruit cracking in litchi and other fruits, especially Sapindaceae plants. View Full-Text
Keywords: Litchi chinensis Sonn.; transcriptome; pericarp; RNA-Seq; cracking Litchi chinensis Sonn.; transcriptome; pericarp; RNA-Seq; cracking
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MDPI and ACS Style

Li, W.-C.; Wu, J.-Y.; Zhang, H.-N.; Shi, S.-Y.; Liu, L.-Q.; Shu, B.; Liang, Q.-Z.; Xie, J.-H.; Wei, Y.-Z. De Novo Assembly and Characterization of Pericarp Transcriptome and Identification of Candidate Genes Mediating Fruit Cracking in Litchi chinensis Sonn.. Int. J. Mol. Sci. 2014, 15, 17667-17685.

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