Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2014, 15(1), 994-1002; doi:10.3390/ijms15010994
Article

Development of Microsatellite Markers in the Branched Broomrape Phelipanche ramosa L. (Pomel) and Evidence for Host-Associated Genetic Divergence

1,* , 2
 and 2
Received: 21 November 2013; in revised form: 7 January 2014 / Accepted: 8 January 2014 / Published: 13 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [192 KB, uploaded 19 June 2014]
Abstract: Phelipanche ramosa is a parasitic plant that infects numerous crops worldwide. In Western Europe it recently expanded to a new host crop, oilseed rape, in which it can cause severe yield losses. We developed 13 microsatellite markers for P. ramosa using next-generation 454 sequencing data. The polymorphism at each locus was assessed in a sample of 96 individuals collected in France within 6 fields cultivated with tobacco, hemp or oilseed rape. Two loci were monomorphic. At the other 11 loci, the number of alleles and the expected heterozygosity ranged from 3 to 6 and from 0.31 to 0.60, respectively. Genetic diversity within each cultivated field was very low. The host crop from which individuals were collected was the key factor structuring genetic variation. Individuals collected on oilseed rape were strongly differentiated from individuals collected on hemp or tobacco, which suggests that P. ramosa infecting oilseed rape forms a genetically diverged race. The microsatellites we developed will be useful for population genetics studies and for elucidating host-associated genetic divergence in P. ramosa.
Keywords: Phelipanche ramosa; parasitic plant; microsatellite markers; genetic diversity; host races Phelipanche ramosa; parasitic plant; microsatellite markers; genetic diversity; host races
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Le Corre, V.; Reibel, C.; Gibot-Leclerc, S. Development of Microsatellite Markers in the Branched Broomrape Phelipanche ramosa L. (Pomel) and Evidence for Host-Associated Genetic Divergence. Int. J. Mol. Sci. 2014, 15, 994-1002.

AMA Style

Le Corre V, Reibel C, Gibot-Leclerc S. Development of Microsatellite Markers in the Branched Broomrape Phelipanche ramosa L. (Pomel) and Evidence for Host-Associated Genetic Divergence. International Journal of Molecular Sciences. 2014; 15(1):994-1002.

Chicago/Turabian Style

Le Corre, Valérie; Reibel, Carole; Gibot-Leclerc, Stéphanie. 2014. "Development of Microsatellite Markers in the Branched Broomrape Phelipanche ramosa L. (Pomel) and Evidence for Host-Associated Genetic Divergence." Int. J. Mol. Sci. 15, no. 1: 994-1002.


Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert