Int. J. Mol. Sci. 2014, 15(1), 1003-1013; doi:10.3390/ijms15011003
Article

Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage

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Received: 19 November 2013; in revised form: 24 December 2013 / Accepted: 9 January 2014 / Published: 14 January 2014
(This article belongs to the Section Bioactives and Nutraceuticals)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: In this study, we examined the protective effect of lactoferrin against DNA damage induced by various hydroxyl radical generation systems. Lactoferrin (LF) was examined with regard to its potential role as a scavenger against radical oxygen species using bovine milk LF. Native LF, iron-saturated LF (holo-LF), and apolactoferrin (apo-LF) effectively suppressed strand breaks in plasmid DNA due to hydroxyl radicals produced by the Fenton reaction. In addition, both native LF and holo-LF clearly protected calf thymus DNA from fragmentation due to ultraviolet irradiation in the presence of H2O2. We also demonstrated a protective effect of all three LF molecules against 8-hydroxydeoxyguanosine (8-OHdG) formation in calf thymus DNA following ultraviolet (UV) irradiation with H2O2. Our results clearly indicate that native LF has reactive oxygen species-scavenging ability, independent of its nature as a masking component for transient metals. We also demonstrated that the protective effect of LF against oxidative DNA damage is due to degradation of LF itself, which is more susceptible to degradation than other bovine milk proteins.
Keywords: lactoferrin; bovine milk; DNA damage; hydroxyl radical; UV irradiation
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MDPI and ACS Style

Ogasawara, Y.; Imase, M.; Oda, H.; Wakabayashi, H.; Ishii, K. Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage. Int. J. Mol. Sci. 2014, 15, 1003-1013.

AMA Style

Ogasawara Y, Imase M, Oda H, Wakabayashi H, Ishii K. Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage. International Journal of Molecular Sciences. 2014; 15(1):1003-1013.

Chicago/Turabian Style

Ogasawara, Yuki; Imase, Megumi; Oda, Hirotsugu; Wakabayashi, Hiroyuki; Ishii, Kazuyuki. 2014. "Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage." Int. J. Mol. Sci. 15, no. 1: 1003-1013.

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