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Int. J. Mol. Sci. 2014, 15(1), 977-993; doi:10.3390/ijms15010977
Article

Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells

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Received: 19 October 2013; in revised form: 20 December 2013 / Accepted: 30 December 2013 / Published: 10 January 2014
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Abstract: The KCNQ gene family, whose members encode Kv7 channels, belongs to the voltage-gated potassium (Kv) channel group. The roles of this gene family have been widely investigated in nerve and muscle cells. In the present study, we investigated several characteristics of Kv7.5, which is strongly expressed in the canine osteosarcoma cell line, CCL-183. Serum starvation upregulated Kv7.5 expression, and the Kv7 channel opener, flupirtine, attenuated cell proliferation by arresting cells in the G0/G1 phase. We also showed that Kv7.5 knockdown helps CCL-183 cells to proliferate. In an effort to find an endogenous regulator of Kv7.5, we used mithramycin A to reduce the level of the transcription factor Sp1, and it strongly inhibited the induction of Kv7.5 in CCL-183 cells. These results suggest that the activation of Kv7.5 by flupirtine may exert an anti-proliferative effect in canine osteosarcoma. Therefore, Kv7.5 is a possible molecular target for canine osteosarcoma therapy.
Keywords: KCNQ5; CCL-183; flupirtine; voltage-gated potassium channels; cell cycle arrest; proliferation KCNQ5; CCL-183; flupirtine; voltage-gated potassium channels; cell cycle arrest; proliferation
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lee, B.H.; Ryu, P.D.; Lee, S.Y. Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells. Int. J. Mol. Sci. 2014, 15, 977-993.

AMA Style

Lee BH, Ryu PD, Lee SY. Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells. International Journal of Molecular Sciences. 2014; 15(1):977-993.

Chicago/Turabian Style

Lee, Bo H.; Ryu, Pan D.; Lee, So Y. 2014. "Serum Starvation-Induced Voltage-Gated Potassium Channel Kv7.5 Expression and Its Regulation by Sp1 in Canine Osteosarcoma Cells." Int. J. Mol. Sci. 15, no. 1: 977-993.


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