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Int. J. Mol. Sci. 2014, 15(1), 186-202; doi:10.3390/ijms15010186
Article

The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray

1,2,†
, 1,2,†
, 1,2,3,* , 1,2
, 1,2
, 1
 and 1,4
1 School of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China 2 Institute of Extreme Environmental Nutrition and Protection, Harbin Institute of Technology, Harbin 150090, China 3 School of Forestry, Northeast Forestry University, Harbin 150026, China 4 School of Food Science and Engineering, Northeast Agriculture University, Harbin 150030, China These authors contributed equally to this work.
* Author to whom correspondence should be addressed.
Received: 24 October 2013 / Revised: 15 December 2013 / Accepted: 18 December 2013 / Published: 24 December 2013
(This article belongs to the collection Advances in Proteomic Research)
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Abstract

Understanding the protection mechanism of 5'-AMP requires comprehensive knowledge of the proteins expressed during the period that the body is exposed to irradiation. Proteomics provides the tools for such analyses. Here, the experimental ICR mice were divided into three groups (normal group, model group and 5'-AMP + irradiation group). After different treatment, the hepatic total protein of each animal in three groups was separated by two-dimensional gel electrophoresis (2-DE). 2-DE analysis revealed fifty-eight protein spots were differentially expressed in comparison to three groups. From 58 protein spots, we selected nine spots to identify by MALDI-TOF-MS and received credible results. They were determined to be type I arginase, annexin A5, regucalcin, catalase, Tpm3 protein, Pdia4 protein, 14-3-3 protein epsilon, NAD-Malate dehydrogenase and heat shock protein 90. Considering the characteristic of these proteins, we proposed a possible protection pathway.
Keywords: 5'-AMP; protective effect; radiation damage; proteomics 5'-AMP; protective effect; radiation damage; proteomics
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Cheng, C.; Zhao, H.; Wang, Z.; Lu, W.; Wang, L.; Wang, R.; Yao, L. The Effect of 5'-Adenylic Acid on Hepatic Proteome of Mice Radiated by 60Co γ-ray. Int. J. Mol. Sci. 2014, 15, 186-202.

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