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A Novel Thylakoid Ascorbate Peroxidase from Jatrophacurcas Enhances Salt Tolerance in Transgenic Tobacco
Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, China
West China School of Pharmacy, Sichuan University, Chengdu 610064, Sichuan, China
Architecture & Environment Department, Sichuan University, Chengdu 610065, Sichuan, China
Department of Civil Engineering, University of British Columbia, Vancouver, BC V6T 1Z4, Canada
* Author to whom correspondence should be addressed.
Received: 16 October 2013; in revised form: 10 December 2013 / Accepted: 13 December 2013 / Published: 24 December 2013
Abstract: Ascorbate peroxidase (APX) plays an important role in the metabolism of hydrogen peroxide in higher plants. In the present study, a novel APX gene (JctAPX) was cloned from Jatropha curcas L. The deduced amino acid sequence was similar to that of APX of some other plant species. JctAPX has a chloroplast transit peptide and was localized to the chloroplasts by analysis with a JctAPX-green fluorescent protein (GFP) fusion protein. Quantitative polymerase chain reaction (qPCR) analysis showed that JctAPX was constitutively expressed in different tissues from J. curcas and was upregulated by NaCl stress. To characterize its function in salt tolerance, the construct p35S: JctAPX was created and successfully introduced into tobacco by Agrobacterium-mediated transformation. Compared with wild type (WT), the transgenic plants exhibited no morphological abnormalities in the no-stress condition. However, under 200 mM NaCl treatment, JctAPX over-expressing plants showed increased tolerance to salt during seedling establishment and growth. In addition, the transgenic lines showed higher chlorophyll content and APX activity, which resulted in lower H2O2 content than WT when subjected to 400 mM NaCl stress. These results suggest that the increased APX activity in the chloroplasts from transformed plants increased salt tolerance by enhancing reactive oxygen species (ROS)-scavenging capacity under short-term NaCl stress conditions.
Keywords: abiotic stress tolerance; anti-oxidative enzymes; ascorbate peroxidase; salt stress; Jatropha curcas
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MDPI and ACS Style
Liu, Z.; Bao, H.; Cai, J.; Han, J.; Zhou, L. A Novel Thylakoid Ascorbate Peroxidase from Jatrophacurcas Enhances Salt Tolerance in Transgenic Tobacco. Int. J. Mol. Sci. 2014, 15, 171-185.
Liu Z, Bao H, Cai J, Han J, Zhou L. A Novel Thylakoid Ascorbate Peroxidase from Jatrophacurcas Enhances Salt Tolerance in Transgenic Tobacco. International Journal of Molecular Sciences. 2014; 15(1):171-185.
Liu, Zhibin; Bao, Han; Cai, Jin; Han, Jun; Zhou, Lirong. 2014. "A Novel Thylakoid Ascorbate Peroxidase from Jatrophacurcas Enhances Salt Tolerance in Transgenic Tobacco." Int. J. Mol. Sci. 15, no. 1: 171-185.