Next Article in Journal
Evaluation of Hepatic Tissue Blood Flow Using Xenon Computed Tomography with Fibrosis Progression in Nonalcoholic Fatty Liver Disease: Comparison with Chronic Hepatitis C
Previous Article in Journal
Lactoferrin Directly Scavenges Hydroxyl Radicals and Undergoes Oxidative Self-Degradation: A Possible Role in Protection against Oxidative DNA Damage
Int. J. Mol. Sci. 2014, 15(1), 1014-1025; doi:10.3390/ijms15011014
Article

Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions

1,* , 1,†
 and
2
1 Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA 2 Pure Circle SDN BHD, Lengkuk Teknologi, 71760 Bandar ENSTEK, Negeri Sembilan, Malaysia Current Address: Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA.
* Author to whom correspondence should be addressed.
Received: 21 November 2013 / Revised: 31 December 2013 / Accepted: 9 January 2014 / Published: 14 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [215 KB, uploaded 19 June 2014]   |  

Abstract

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) & 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
Keywords: rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).
SciFeed

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
RIS
MDPI and ACS Style

Prakash, I.; Chaturvedula, V.S.P.; Markosyan, A. Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions. Int. J. Mol. Sci. 2014, 15, 1014-1025.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert