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Int. J. Mol. Sci. 2014, 15(1), 1014-1025; doi:10.3390/ijms15011014

Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions

1
Organic Chemistry Department, The Coca-Cola Company, Global Research and Development, One Coca-Cola Plaza, Atlanta, GA 30313, USA
2
Pure Circle SDN BHD, Lengkuk Teknologi, 71760 Bandar ENSTEK, Negeri Sembilan, Malaysia
Current Address: Natural Ingredient Development, Blue California, 30111 Tomas, Rancho Santa Margarita, CA 92688, USA.
*
Author to whom correspondence should be addressed.
Received: 21 November 2013 / Revised: 31 December 2013 / Accepted: 9 January 2014 / Published: 14 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) & 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
Keywords: rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Prakash, I.; Chaturvedula, V.S.P.; Markosyan, A. Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions. Int. J. Mol. Sci. 2014, 15, 1014-1025.

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