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Int. J. Mol. Sci. 2014, 15(1), 1014-1025; doi:10.3390/ijms15011014
Article

Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions

1,* , 1,†
 and
2
Received: 21 November 2013 / Revised: 31 December 2013 / Accepted: 9 January 2014 / Published: 14 January 2014
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Degradation of rebaudioside M, a minor sweet component of Stevia rebaudiana Bertoni, under conditions that simulated extreme pH and temperature conditions has been studied. Thus, rebaudioside M was treated with 0.1 M phosphoric acid solution (pH 2.0) and 80 °C temperature for 24 h. Experimental results indicated that rebaudioside M under low pH and higher temperature yielded three minor degradation compounds, whose structural characterization was performed on the basis of 1D (1H-, 13C-) & 2D (COSY, HSQC, HMBC) NMR, HRMS, MS/MS spectral data as well as enzymatic and acid hydrolysis studies.
Keywords: rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization rebaudioside M; stability; pH; temperature; degradation products; spectroscopic data; structure characterization
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Prakash, I.; Chaturvedula, V.S.P.; Markosyan, A. Structural Characterization of the Degradation Products of a Minor Natural Sweet Diterpene Glycoside Rebaudioside M under Acidic Conditions. Int. J. Mol. Sci. 2014, 15, 1014-1025.

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